Key Product Details

Species Reactivity

Human

Applications

Intracellular Staining by Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 1031751

View all Perforin Antibodies »

Product Specifications

Immunogen

Chinese Hamster Ovary cell line CHO-derived human Perforin
Pro22-Trp555
Accession # P14222

Specificity

Detects human Perforin in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Perforin Antibody

Detection of Perforin in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with (A) Mouse Anti-Human Perforin Monoclonal Antibody (Catalog # MAB103853) or (B) isotype control antibody (MAB002) followed by Anti-Mouse IgG Allophycocyanin-conjugated Secondary Antibody (F0101B) and Mouse anti-Human CD56 Phycoerythrin-conjugated Monoclonal Antibody (FAB24086P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (FC012). Staining was performed using our Staining Intracellular Molecules protocol.

Applications for Human Perforin Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

0.25 µg/mL
Sample: Human PBMC fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Perforin

Perforin is a pore forming, cytolytic protein that is a component of cytolytic T-cell and NK cell granules.  Perforin activates clathrin- and dynamin-dependent endocytosis, which removes perforin and granzymes from the plasm membrane to early endosomes, preserving outer membrane integrity.  Perforin facilitates granzyme uptake and avoids the proinflammatory necrotic death of a membrane-damaged cell.  Defects in the Perforin gene results in Familial hemophagocytic lymphohistiocytosis (FHL).  Perforin-based effector systems are involved not only in the lysis of abnormal cells but also in the down-regulation of cellular immune activation.

Long Name

Perforin 1 (Pore Forming Protein)

Alternate Names

Cytolysin, FLH2, HPLH2, P1, PFP, PRF1

Entrez Gene IDs

5551 (Human); 18646 (Mouse); 50669 (Rat)

Gene Symbol

PRF1

UniProt

P14222

Additional Perforin Products

Product Documents for Human Perforin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Perforin Antibody

For research use only

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