Human peripheral blood mononuclear cells (PBMCs) were stained with (A) Mouse Anti-Human Perforin Monoclonal Antibody (Catalog # MAB103853) or (B) isotype control antibody (MAB002) followed by Anti-Mouse IgG Allophycocyanin-conjugated Secondary Antibody (F0101B) and Mouse anti-Human CD56 Phycoerythrin-conjugated Monoclonal Antibody (FAB24086P). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (FC012). Staining was performed using our Staining Intracellular Molecules protocol.

Applications for Human Perforin Antibody

Application

Recommended Usage

Intracellular Staining by Flow Cytometry

0.25 µg/mL
Sample: Human PBMC fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Perforin

Perforin is a pore forming, cytolytic protein that is a component of cytolytic T-cell and NK cell granules. Perforin activates clathrin- and dynamin-dependent endocytosis, which removes perforin and granzymes from the plasm membrane to early endosomes, preserving outer membrane integrity. Perforin facilitates granzyme uptake and avoids the proinflammatory necrotic death of a membrane-damaged cell. Defects in the Perforin gene results in Familial hemophagocytic lymphohistiocytosis (FHL). Perforin-based effector systems are involved not only in the lysis of abnormal cells but also in the down-regulation of cellular immune activation.

Long Name

Perforin 1 (Pore Forming Protein)

Alternate Names

Cytolysin, FLH2, HPLH2, P1, PFP, PRF1

Entrez Gene IDs

5551 (Human); 18646 (Mouse); 50669 (Rat)

Gene Symbol

PRF1

UniProt

P14222

Additional Perforin Products

Product Documents for Human Perforin Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Perforin Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Perforin Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB103853

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Perforin Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Perforin Antibody

Detection of Perforin in Human PBMCs by Flow Cytometry.