Human Peroxiredoxin 1 Antibody

Western Blot Shows Human Peroxiredoxin 1 Specificity Using Knockout Cell Line.

Western blot shows lysates of U2OS human osteosarcoma cell line and Peroxiredoxin 1 knockout U2OS cell line (KO). Nitrocellulose membrane was probed with 0.5 µg/mL of Mouse Anti-Human Peroxiredoxin 1 Monoclonal Antibody (Catalog # MAB3488) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody. A specific band was detected for Peroxiredoxin 1 at approximately 22 kDa (as indicated) in the parental U2OS cell line, but is not detectable in knockout U2OS cell line. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

Detection of Peroxiredoxin-1/PRDX1 by Immunoprecipitation.

Immunoprecipitation was performed on cell lysate of U2OS human osteosarcoma cell line using 1.0 μg of Mouse Anti-Human PRDX1 Monoclonal Antibody (Catalog # MAB3488) pre-coupled to protein G or protein A beads. Immunoprecipitated PRDX1 was detected with a Goat Anti-PRDX1 Polyclonal Antibody. The Ponceau stained transfers of each blot are shown. SM=10% starting material; UB=10% unbound fraction; IP=immunoprecipitated. Image, protocol, and testing courtesy of YCharOS Inc. (ycharos.com).