Human Peroxiredoxin 4 Antibody

Detection of Human Peroxiredoxin 4 by Western Blot.

Western blot shows lysates of HEK293 human embryonic kidney cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Peroxiredoxin 4 Monoclonal Antibody (Catalog # MAB5460) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Peroxiredoxin 4 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Peroxiredoxin 4 in HeLa Human Cell Line.

Peroxiredoxin 4 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Peroxiredoxin 4 Monoclonal Antibody (Catalog # MAB5460) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Peroxiredoxin 4 in Human Liver Cancer Tissue.

Peroxiredoxin 4 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Mouse Anti-Human Peroxiredoxin 4 Monoclonal Antibody (Catalog # MAB5460) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.