Human Phospho-CROCC (S1476) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB11335
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2734A
Product Specifications
Immunogen
Phosphopeptide containing the human CROCC S site 1476
Accession # Q5TZA2
Accession # Q5TZA2
Specificity
Detects Human Phospho-CROCC in direct ELISA
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human Phospho-CROCC (S1476) Antibody
Detection of Human CROCC by Western Blot.
Western blot shows lysates of MDA-MB-231 human breast cancer cells and MDA-MB-231 + Calyculin A. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human CROCC Monoclonal Antibody (Catalog # MAB11335) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for CROCC at approximately ~228 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Detection of CROCC in HEL 92.1.7 and Daudi cells (both positive).
CROCC was detected in immersion fixed HEL 92.1.7 Human Erythroleukemic Cells and Daudi Human Burkitt's Lymphoma Cells (Both Positive) using Rabbit Anti-Human CROCC Monoclonal Antibody (Catalog # MAB11335) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human Phospho-CROCC (S1476) Antibody
Application
Recommended Usage
Immunocytochemistry
3-15 µg/mL
Sample: Immersion fixed HEL 92.1.7 Human Erythroleukemic Cells and Daudi Human Burkitt's Lymphoma Cells (Both Positive)
Sample: Immersion fixed HEL 92.1.7 Human Erythroleukemic Cells and Daudi Human Burkitt's Lymphoma Cells (Both Positive)
Western Blot
1 µg/mL
Sample: MDA-MB-231 human breast cancer cells and MDA-MB-231 + Calyculin A
Sample: MDA-MB-231 human breast cancer cells and MDA-MB-231 + Calyculin A
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CROCC
Long Name
Ciliary Rootlet Coiled-Coil, Rootletin
Alternate Names
CROCC1, KIAA0445, ROLT, TAX1BP2
Entrez Gene IDs
9696 (Human)
Gene Symbol
CROCC
UniProt
Additional CROCC Products
Product Documents for Human Phospho-CROCC (S1476) Antibody
Product Specific Notices for Human Phospho-CROCC (S1476) Antibody
For research use only
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