Human Phospho-IKK alpha (S176/S180) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3768
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 829177
Product Specifications
Immunogen
Phosphopeptide containing the human IKK alpha S176/S180
Accession # O15111
Accession # O15111
Specificity
Detects human IKK alpha when phosphorylated at S176/S180.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Human Phospho-IKK alpha (S176/S180) Antibody
Detection of Human Phospho-IKK alpha (S176/S180) by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 100 nM Calyculin A (Catalog # 1336) and 20 ng/mL Recombinant Human TNF-a (Catalog # 210-TA) for 10 minutes. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human Phospho-IKKa (S176/S180) Mono-clonal Antibody (Catalog # MAB3768) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-IKKa (S176/S180) at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.Applications for Human Phospho-IKK alpha (S176/S180) Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line treated with Calyculin A and Recombinant Human TNF‑ alpha (Catalog # 210-TA)
Sample: HeLa human cervical epithelial carcinoma cell line treated with Calyculin A and Recombinant Human TNF‑ alpha (Catalog # 210-TA)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IKK alpha
I kappaB kinase alpha (IKK alpha) is also known as IKBKA, CHUK, and IKK1. The classical active IKK complex, composed of IKK alpha, IKK beta, and two forms of processed IKK gamma, phosphorylates and inactivates I kappaB, resulting in the release and nuclear translocation of active NF-kappa B. IKK alpha contains kinase, leucine zipper, and helix-loop-helix domains, and heterodimerizes with IKK beta. NF-kappa B-inducing kinase (NIK) phosphorylates and activates IKK alpha.
Long Name
IkB Kinase alpha
Alternate Names
CHUK, IkBKalpha, IKK1, NFKBIKA, TCF16
Gene Symbol
CHUK
UniProt
Additional IKK alpha Products
Product Documents for Human Phospho-IKK alpha (S176/S180) Antibody
Product Specific Notices for Human Phospho-IKK alpha (S176/S180) Antibody
For research use only
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