Human Phospho-MSPR/Ron (Y1238/Y1239) Antibody

Detection of Human Phospho-MSP R/Ron (Y1238/Y1239) by Western Blot.

Western blot shows lysates of MDA-MB-453 human breast cancer cell line untreated (-) or treated (+) with 100 µM pervanadate (PV) for 10 minutes. The membranes were untreated or treated with calf intestinal phosphatase (CIP). PVDF membrane was probed with 1 µg/mL of Human Phospho-MSP R/Ron (Y1238/Y1239) Antigen Affinity-purified Polyclonal Antibody, followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-MSP R/Ron (Y1238/Y1239) at approximately 170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Phospho-MSP R/Ron (Y1238/Y1239) by Western blot.

Human Phospho-MSP R/Ron (Y1238/Y1239) was immunoprecipitated from 1 mg of lysates of MDA-MB-453 human breast cancer cell line untreated (-) or treated (+) with 100 uM Pervanadate (PV) for 10 minutes or 100 ng/mL Recombinant Human MSP/MST1 Protein (Catalog # 352-MS) for 10 minutes. MSP R/Ron (Y1238/Y1239) was immunoprecipiated with 1 ug Goat Anti-Human MSP R/Ron Antigen Affinity-purified Polyclonal Antibody(Catalog # AF691). The MSP R/Ron (Y1238/Y1239)-antibody complexes were absorbed using Protein G Sepharose. Immunoprecipitated MSP R/Ron (Y1238/Y1239) was detected by Western blot using 0.1 µg/mL of Rabbit Anti-Human Phospho-MSP R/Ron (Y1238/Y1239) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1947).

Detection of Human Human Phospho-MSPR/Ron (Y1238/Y1239) Antibody by Western Blot

The expression and activity of Ron receptor isoforms in primary tumors and high-grade serous ovarian (HG-SOC) PDXsA. Upper panel displays western blot analysis of tumor lysates from patients assayed for phosphorylated (active) sfRon and Ron. Lower panel shows western blot analysis of total levels of Ron isoforms. The higher molecular weight sfRon bands (sfRon-HMW), which are putative, posttranslationally modified sfRon forms were also noted. The blots were stripped and re-probed for beta-actin. Lanes represent: healthy ovary (1, 2, 3); ovarian adenocarcinoma (4); carcinosarcoma (5); endometrioid adenocarcinoma (6) and HG-SOC (7). B. Upper panel displays western blot analysis of tumor lysates from HG-SOC PDXs assayed for phosphorylated Ron isoforms. Lower panel shows western blot analysis of total levels of Ron isoforms. The blots were stripped and re-probed for beta-actin or GAPDH. C. The expression of Ron isoforms was assessed by WES capillary electrophoresis-based protein assay in OVCAR3-sfRon cell line engineered to express sfRon vs. parental OVACR3 cells and compared with sfRon positive or negative HG-SOC PDXs. GAPDH was used as loading control. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27551332), licensed under a CC-BY license. Not internally tested by R&D Systems.