Human Phospho-p53 (S15) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB18391
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
CyTOF-ready, Immunocytochemistry, Intracellular Staining by Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 261366
Product Specifications
Immunogen
Phosphopeptide containing the human p53 S15 site
Accession # P04637
Accession # P04637
Specificity
Detects human p53 phosphorylated at S15. No cross-reactivity with human p53 that is unphosphorylated at S15 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Phospho-p53 (S15) Antibody
Detection of Phospho-p53 in camptothecin-treated MCF‑7 Human Cell Line by Flow Cytometry.
MCF-7 human breast cancer cell line was unstimulated (light orange filled histogram) or treated with 1 µM camptothecin for 6 hours (dark orange filled histogram), then stained with Mouse Anti-Human Phospho-p53 (S15) Monoclonal Antibody (Catalog # MAB18391) or isotype control (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.Applications for Human Phospho-p53 (S15) Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed U2OS human osteosarcoma cell line
Sample: Immersion fixed U2OS human osteosarcoma cell line
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Sample: MCF‑7 human breast cancer cell line treated with camptothecin fixed with paraformaldehyde and permeabilized with saponin
Sample: MCF‑7 human breast cancer cell line treated with camptothecin fixed with paraformaldehyde and permeabilized with saponin
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: p53
The p53 tumor suppressor protein acts to enforce cell cycle checkpoints or signal apoptosis in cells that have incurred genotoxic damage. The ATM or ATR kinases can phosphorylate p53 at serine 15 to enforce cell cycle arrest. Serine 15 phosphorylation leads to p53 stabilization and enhances transactivation of p53 target genes.
Alternate Names
BCC7, LFS1, TP53, TRP53
Gene Symbol
TP53
UniProt
Additional p53 Products
Product Documents for Human Phospho-p53 (S15) Antibody
Product Specific Notices for Human Phospho-p53 (S15) Antibody
For research use only
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