Human Phospho-p53 (S392) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2996
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 2185A
Product Specifications
Immunogen
Phosphopeptide containng the human p53 S392 site
Accession # P04637
Accession # P04637
Specificity
Detects human p53 when phoshorylated at S392
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human Phospho-p53 (S392) Antibody
Detection of Human Phospho-p53 (S392) by Western Blot.
Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 1uM Camptothecin (CPT) for 5 hours. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human Phospho-p53 (S392) Monoclonal Antibody (Catalog # MAB2996) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-p53 (S392) at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Phospho-p53 (S392) in MCF‑7 Human Cell Line.
p53 phosphorylated at S392 was detected in immersion fixed MCF-7 human breast cancer cell line using Rabbit Anti-Human Phospho-p53 (S392) Monoclonal Antibody (Catalog # MAB2996) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Phospho-p53 (S392) Antibody
Application
Recommended Usage
Immunocytochemistry
3-25 µg/mL
Sample: Immersion fixed MCF‑7 human breast cancer cell line
Sample: Immersion fixed MCF‑7 human breast cancer cell line
Western Blot
2 µg/mL
Sample: MCF-7 human breast cancer cell line
Sample: MCF-7 human breast cancer cell line
Reviewed Applications
Read 1 review rated 5 using MAB2996 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: p53
Alternate Names
BCC7, LFS1, TP53, TRP53
Gene Symbol
TP53
UniProt
Additional p53 Products
Product Documents for Human Phospho-p53 (S392) Antibody
Product Specific Notices for Human Phospho-p53 (S392) Antibody
For research use only
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