Human Phospho-p70 S6 Kinase (T229) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB8964
Recombinant Monoclonal Antibody.
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Mouse
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1015B
Product Specifications
Immunogen
Phosphopeptide containing human p70S6K T229
Specificity
Detects human p70 S6 Kinase when phosphorylated at T229.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human Phospho-p70 S6 Kinase (T229) Antibody
Detection of Human Phospho-p70 S6 Kinase (T229) by Western Blot.
Western blot shows lysates of MCF-7 human breast cancer cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IGF-I (Catalog # 291-G1) for 1 hour. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-p70 S6 Kinase (T229) Monoclonal Antibody (Catalog # MAB8964) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-p70 S6 Kinase (T229) at approximately 70 and 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Phospho-p70 S6 Kinase (T229) in MCF‑7 Human Cell Line.
p70 S6 Kinase phosphorylated at T229 was detected in immersion fixed MCF-7 human breast cancer cell line treated with 100 ng/mL Recombinant Human IGF-I (Catalog # 291-G1) using Rabbit Anti-Human Phospho-p70 S6 Kinase (T229) Monoclonal Antibody (Catalog # MAB8964) at 2.5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Phospho-p70 S6 Kinase (T229) Antibody
Application
Recommended Usage
Immunocytochemistry
2.5-25 µg/mL
Sample: Immersion fixed MCF‑7 human breast cancer cell line treated with 100 ng/mL Recombinant Human IGF-I (Catalog # 291-G1)
Sample: Immersion fixed MCF‑7 human breast cancer cell line treated with 100 ng/mL Recombinant Human IGF-I (Catalog # 291-G1)
Western Blot
0.5 µg/mL
Sample: MCF‑7 human breast cancer cell line treated with Recombinant Human IGF‑I (Catalog # 291-G1)
Sample: MCF‑7 human breast cancer cell line treated with Recombinant Human IGF‑I (Catalog # 291-G1)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: p70 S6 Kinase
References
- Ferrari, S. et al. (1994) Crit. Rev. Biochem. Mol. Biol. 29:385.
- Edelmann, H.M. et al. (1996) J. Biol. Chem. 271:963.
Alternate Names
p70-alpha, RPS6KB1, S6K1, STK14A
Gene Symbol
RPS6KB1
Additional p70 S6 Kinase Products
Product Documents for Human Phospho-p70 S6 Kinase (T229) Antibody
Product Specific Notices for Human Phospho-p70 S6 Kinase (T229) Antibody
For research use only
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