Human Phospho-Paxillin (Y118) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6164
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 601327
Product Specifications
Immunogen
Phosphopeptide containing the human Paxillin Y118 site
Accession # P49023
Accession # P49023
Specificity
Detects Phospho-Paxillin (Y118) in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Phospho-Paxillin (Y118) Antibody
Detection of Human Phospho-Paxillin (Y118) by Western Blot.
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with Pervanadate for 5 minutes. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Phospho-Paxillin (Y118) Antigen Affinity-purified Monoclonal Antibody (Catalog # MAB6164) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phospho-Paxillin (Y118) at approximately 80 kDa (as indicated). For additional reference, the membran was stripped and reprobed with 2 µg/mL of Sheep Anti-Human/Mouse/Rat Paxillin Antigen Affinity-purified Polyclonal Antibody (lowerpanel, Catalog # AF4259). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Phospho-Paxillin (Y118) Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line and Jurkat human acute T cell leukemia cell line treated with Pervanadate
Sample: K562 human chronic myelogenous leukemia cell line and Jurkat human acute T cell leukemia cell line treated with Pervanadate
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
For liquid material, refer to CoA for concentration.
Formulation
Supplied as a 0.2 μm filtered solution in PBS. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after opening.
- 6 months, -20 to -70 °C under sterile conditions after opening.
Background: Paxillin
Alternate Names
PXN
Gene Symbol
PXN
UniProt
Additional Paxillin Products
Product Documents for Human Phospho-Paxillin (Y118) Antibody
Product Specific Notices for Human Phospho-Paxillin (Y118) Antibody
For research use only
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