Human Phospho-Progesterone R/NR3C3 (S294) Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5955
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Product Specifications
Immunogen
Phosphopeptide containing human Progesterone RB S294 site
Accession # P06401
Accession # P06401
Specificity
Detects human Phospho-Progesterone R/NR3C3 (S294) in Western blots.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human Phospho-Progesterone R/NR3C3 (S294) Antibody
Detection of Human Phospho-Progesterone RB (S294) and Phospho-Progesterone RA (S130) by Western Blot.
Western blot shows lysates of T47D human breast cancer cell line untreated (-) or treated (+) with 100 nM synthetic progestin promegestone (R5020) for 60 minutes. PVDF Membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-Progesterone R/NR3C3 (S294) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5955) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-Progesterone RB (S294) at approximately 118 kDa and Phospho-Progesterone RA (S130) at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Phospho-Progesterone R/NR3C3 (S294) Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: T47D human breast cancer cell line
Sample: T47D human breast cancer cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Progesterone R/NR3C3
Progesterone Receptor B (PR-B) is a 118 kDa member of the NR3 subfamily within the nuclear hormone receptor family of proteins. It is expressed in female reproductive tissues as well as neurons throughout the CNS. PR-B is particularly important in the mammary gland where it mediates proliferative responses to progesterone. Human PR‑B is 933 amino acids (aa) in length. It contains an N-terminal regulatory region (aa 1‑566), a DNA binding domain (aa 567‑639), and a steroid‑binding region (aa 681‑933). Ligand binding induces a key phosphorylation event at Ser294 catalyzed by ERK1/2, regulating cell growth, cell adhesion, and gene transcription. An alternate start site at Met165 generates 90 kDa PR-A, an isoform particularly important in the ovary and uterus that insures fertility.
Long Name
Progesterone Receptor
Alternate Names
NR3C3, PGR, ProgesteroneR
Entrez Gene IDs
5241 (Human)
Gene Symbol
PGR
UniProt
Additional Progesterone R/NR3C3 Products
Product Documents for Human Phospho-Progesterone R/NR3C3 (S294) Antibody
Product Specific Notices for Human Phospho-Progesterone R/NR3C3 (S294) Antibody
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...