Human Phospho-PYK2/FAK2 (Y402) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6210
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 592918
Product Specifications
Immunogen
Phosphopeptide containing the human PYK2/FAK2 Y402 site
Accession # Q14289
Accession # Q14289
Specificity
Detects human PYK2/FAK2 when phosphorylated at Y402.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Phospho-PYK2/FAK2 (Y402) Antibody
Detection of HumanPhospho-PYK2/FAK2 (Y402) by Western Blot.
Western blot shows lysates of Raji human Burkitt's lymphoma cell line and Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 30 minutes and 10 µg/mL Mouse Anti-Human CD3e Monoclonal Antibody (Catalog # MAB100) for 15 minutes. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Phospho-PYK2/FAK2 (Y402) Monoclonal Antibody (Catalog # MAB6210) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Phospho-PYK2/FAK2 (Y402) at approximately 105-115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Phospho-PYK2/FAK2 (Y402) by Simple WesternTM.
Simple Western lane view shows lysates of Raji human Burkitt's lymphoma cell line untreated (-) or treated (+) with 0.2 mg/mL Pervanadate (PV) for 30 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-PYK2/FAK2 (Y402) at approximately 113 kDa (as indicated) using 5 µg/mL of Mouse Anti-Human Phospho-PYK2/FAK2 (Y402) Monoclonal Antibody (Catalog # MAB6210). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human Phospho-PYK2/FAK2 (Y402) Antibody
Application
Recommended Usage
Simple Western
5 µg/mL
Sample: Raji human Burkitt's lymphoma cell line treated with Pervanadate (PV)
Sample: Raji human Burkitt's lymphoma cell line treated with Pervanadate (PV)
Western Blot
0.5 µg/mL
Sample: Raji human Burkitt's lymphoma cell line and Jurkat human acute T cell leukemia cell line treated with Pervanadate (PV) or Mouse Anti-Human CD3 epsilon Monoclonal Antibody (Catalog # MAB100)
Sample: Raji human Burkitt's lymphoma cell line and Jurkat human acute T cell leukemia cell line treated with Pervanadate (PV) or Mouse Anti-Human CD3 epsilon Monoclonal Antibody (Catalog # MAB100)
Reviewed Applications
Read 5 reviews rated 4.2 using MAB6210 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PYK2/FAK2
PYK2 (Proline-rich kinase 2; also Ptk2b, Fak2, RAFTK and CAK beta) is a 112‑116 kDa member of the Fak subfamily, tyrosine protein kinase family. It is expressed in multiple cell types, including endothelial cells, vascular smooth muscle cells, megakaryocytes and neurons. PYK2 is activated by elevated intracellular Ca++ and is associated with MAPK pathway activation. Human PYK2 is 1009 amino acids (aa) in length. PYK2 phosphorylation at Tyr402 is associated with enzymatic activation, intercellular localization, cell growth, cell motility, and regulating molecular associations. Over aa 390‑410, human PYK2 is 100% aa identical to mouse PYK2.
Long Name
Proline-rich Tyrosine Kinase 2
Alternate Names
CADTK, CAKB, FADK2, FAK2, FRNK, PTK2B, RAFTK
Gene Symbol
PTK2B
UniProt
Additional PYK2/FAK2 Products
Product Documents for Human Phospho-PYK2/FAK2 (Y402) Antibody
Product Specific Notices for Human Phospho-PYK2/FAK2 (Y402) Antibody
For research use only
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