Human Phospho-Ribosomal Protein S6/RPS6 (S235/S236) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB54361
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 973210
Product Specifications
Immunogen
Phosphopeptide containing human Ribosomal Protein S6 S235/S236 sites
Accession # P62753
Accession # P62753
Specificity
Detects human Ribosomal Protein S6 when dually phosphorylated at S235 and S236.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Human Phospho-Ribosomal Protein S6/RPS6 (S235/S236) Antibody
Detection of Human Phospho-Ribosomal Protein S6/RPS6 (S235/S236) by Western Blot.
Western blot shows lysates of HEK293T human embryonic kidney cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human EGF (Catalog # 236-EG) for 30 minutes. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human Phospho-Ribosomal Protein S6/RPS6 (S235/S236) Monoclonal Antibody (Catalog # MAB54361) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-Ribosomal Protein S6/RPS6 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Phospho-Ribosomal Protein S6/RPS6 (S235/S236) in MCF‑7 Human Cell Line.
Ribosomal Protein S6/RPS6 was detected in immersion fixed MCF-7 human breast cancer cell line untreated or treated with Rapamycin using Rat Anti-Human Phospho-Ribosomal Protein S6/RPS6 (S235/S236) Monoclonal Antibody (Catalog # MAB54361) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Phospho-Ribosomal Protein S6/RPS6 (S235/S236) Antibody
Application
Recommended Usage
Immunocytochemistry
3-25 µg/mL
Sample: Immersion fixed MCF-7 human breast cancer cell line
Sample: Immersion fixed MCF-7 human breast cancer cell line
Western Blot
1 µg/mL
Sample: HEK293T human embryonic kidney cell line treated with Recombinant Human EGF (Catalog # 236-EG)
Sample: HEK293T human embryonic kidney cell line treated with Recombinant Human EGF (Catalog # 236-EG)
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Ribosomal Protein S6/RPS6
40S Ribosomal Protein S6 (RPS6) is the major substrate of protein kinases, particularly p70 S6 kinase, in eukaryotic ribosomes. RPS6 phosphorylation at S235, S236, S240, and S244 upregulates the translation of mRNAs containing an oligopyrimidine tract at their transcriptional start sites. This phosphorylation is stimulated by growth factors, tumor promoting agents, and other mitogens. RPS6 is dephosphorylated during growth arrest.
Alternate Names
RPS6
Gene Symbol
RPS6
UniProt
Additional Ribosomal Protein S6/RPS6 Products
Product Documents for Human Phospho-Ribosomal Protein S6/RPS6 (S235/S236) Antibody
Product Specific Notices for Human Phospho-Ribosomal Protein S6/RPS6 (S235/S236) Antibody
For research use only
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