Human Phospho-STAT6 (Y641) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB37171
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 943008
Product Summary for Human Phospho-STAT6 (Y641) Antibody
Immunogen
Phosphopeptide containing the human STAT6 Y641 site
Accession # P42226
Accession # P42226
Specificity
Detects human STAT6 when phosphorylated at Y641 in Western blots.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Human Phospho-STAT6 (Y641) Antibody
Detection of Human Phospho-STAT6 by Western Blot.
Western blot shows lysates of U937 human histiocytic lymphoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IL-4 (Catalog # 204-IL) for 15 minutes. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human Phospho-STAT6 (Y641) Monoclonal Antibody (Catalog # MAB37171) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for STAT6 at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Phospho-STAT6 (Y641) Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: U937 human histiocytic lymphoma cell line treated with Recombinant Human IL‑4 (Catalog # 204-IL)
Sample: U937 human histiocytic lymphoma cell line treated with Recombinant Human IL‑4 (Catalog # 204-IL)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: STAT6
Long Name
Signal Transducer and Activator of Transcription 6
Alternate Names
D12S1644, EC 2.4.1.227, EC 2.7.7.6, IL-4 Stat, IL-4-STAT, signal transducer and activator of transcription 6, signal transducer and activator of transcription 6, interleukin-4 induced, STAT, interleukin-4 induced, STAT, interleukin4-induced, STAT6B, STAT6C, transcription factor IL-4 STAT
Gene Symbol
STAT6
UniProt
Additional STAT6 Products
Product Documents for Human Phospho-STAT6 (Y641) Antibody
Product Specific Notices for Human Phospho-STAT6 (Y641) Antibody
For research use only
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