Human Phospho-SYK (Y525/Y526) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6459
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 616268
Product Specifications
Immunogen
Phosphopeptide containing human SYK Y525/Y526 sites
Specificity
Detects human Phospho-SYK when phosphorylated at Y525/Y526 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Phospho-SYK (Y525/Y526) Antibody
Detection of Human Phospho-SYK (Y525/Y526) by Western Blot.
Western blot shows lysates of Ramos human Burkitt's lymphoma cell line untreated (-) or treated (+) with 10 µg/mL IgM for 2 minutes and U937 human histiocytic lymphoma cell line untreated (-) or treated (+) with 2nM pervanadate (PV) for 5 minutes. PVDF Membrane was probed with 0.1 µg/mL of Human Phospho-SYK (Y525/Y526) Monoclonal Antibody (Catalog # MAB6459) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phopho-SYK (Y525/Y526) at approximately 80 kDa (as indicated). For additional reference, the membrane was stripped and reprobed with mouse anti-SYK (lower panel). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Phospho-SYK (Y525/Y526) Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Ramos human Burkitt's lymphoma cell line treated with IgM and U937 human histiocytic lymphoma cell line treated with pervanadate
Sample: Ramos human Burkitt's lymphoma cell line treated with IgM and U937 human histiocytic lymphoma cell line treated with pervanadate
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SYK
Spleen tyrosine kinase (SYK; also tyrosine-protein kinase SYK) is a 70-80 kDa, ubiquitously expressed member of the protein kinase superfamily, the tyrosine protein kinase family, and the SYK/ZAP-70 subfamily of proteins. Human SYK is 635 amino acids (aa) in length and contains two SH2 domains (aa 14-106 and aa 168-259), and one protein kinase domain (aa 371-631). A splicing variant produces a second isoform that has a deletion of aa 371-631 found in the longer isoform. Human SYK shares 92% and 91% aa sequence identity with mouse and rat SYK, respectively. Functionally, SYK is a positive effector of B-cell antigen receptor-stimulated responses.
Long Name
Spleen Tyrosine Kinase
Alternate Names
DKFZp313N1010, EC 2.7.10, EC 2.7.10.2, FLJ25043, spleen tyrosine kinaseFLJ37489, tyrosine-protein kinase SYK
Gene Symbol
SYK
Additional SYK Products
Product Documents for Human Phospho-SYK (Y525/Y526) Antibody
Product Specific Notices for Human Phospho-SYK (Y525/Y526) Antibody
For research use only
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