Human Phospho-WNK1 (T60) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4720
Recombinant Monoclonal Antibody.
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1259F
Product Specifications
Immunogen
Phosphopeptide containing human WNK1 T60 site
Accession # Q9H4A3
Accession # Q9H4A3
Specificity
Detects human WNK1 when phosphorylated at T60 in Western blots.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human Phospho-WNK1 (T60) Antibody
Detection of Human Phospho-WNK1 (T60) by Western Blot.
Western blot shows lysates of HEK293 human embryonic kidney cell line untreated (-) or treated (+) with 1 µg/mL insulin for 5 minutes. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human Phospho-WNK1 (T60) Monoclonal Antibody (Catalog # MAB4720) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-WNK1 (T60) at approximately 280 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Phospho-WNK1 (T60) Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HEK293 human embryonic kidney cell line treated with insulin
Sample: HEK293 human embryonic kidney cell line treated with insulin
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: WNK1
With No Lysine (K) 1 (WNK1) belongs to a novel family of serine/threonine protein kinases involved in regulating ion transport. The WNKs contain a cysteine instead of the usual lysine in their kinase activation domains. WNK1 mutations have been implicated in Pseudohypoaldosteronism type II (PHAII), an autosomal dominant disorder characterized by hypertension, increased renal salt absorption, and impaired potassium and hydrogen excretion. WNK1 is phosphorylated by Akt1 and Akt2 at T60, and may function as a negative regulator of insulin-stimulated mitogenesis.
Long Name
WNK Lysine-deficient Protein Kinase 1
Alternate Names
KDP, PHA2C, PRKWNK1
Gene Symbol
WNK1
UniProt
Additional WNK1 Products
Product Documents for Human Phospho-WNK1 (T60) Antibody
Product Specific Notices for Human Phospho-WNK1 (T60) Antibody
For research use only
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