Human PI 3-Kinase p85 beta Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6777
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 572001
Product Specifications
Immunogen
E. coli-derived recombinant human PI 3‑Kinase p85 beta
Lys516-Gly642
Accession # O00459
Lys516-Gly642
Accession # O00459
Specificity
Detects human PI 3‑Kinase p85 beta in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant
human (rh) PI 3-Kinase p85 beta (aa 325-428), rhPI 3-Kinase p85 alpha, rhPI
3-Kinase p87, rhPIK3R4, or rhPIK3R5 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human PI 3-Kinase p85 beta Antibody
Detection of Human PI 3-Kinase p85 beta by Western Blot.
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human PI 3-Kinase p85 beta Monoclonal Antibody (Catalog # MAB6777) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PI 3-Kinase p85 beta at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
PI 3‑Kinase p85 beta in K562 Human Cell Line.
PI 3-Kinase p85 beta was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Mouse Anti-Human PI 3-Kinase p85 beta Monoclonal Antibody (Catalog # MAB6777) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human PI 3-Kinase p85 beta Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed K562 human chronic myelogenous leukemia cell line
Sample: Immersion fixed K562 human chronic myelogenous leukemia cell line
Western Blot
2 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line
Sample: K562 human chronic myelogenous leukemia cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PI 3-Kinase p85 beta
Long Name
Phosphatidylinositol-3-kinase 85 kDa Regulatory Subunit beta
Alternate Names
p85-beta, PI 3Kinase p85 beta, PIK3R2
Gene Symbol
PIK3R2
UniProt
Additional PI 3-Kinase p85 beta Products
Product Documents for Human PI 3-Kinase p85 beta Antibody
Product Specific Notices for Human PI 3-Kinase p85 beta Antibody
For research use only
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