Human Plexin A1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6536
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 659807
Product Specifications
Immunogen
E. coli-derived recombinant human Plexin A1
Asn986-Leu1152
Accession # Q9UIW2
Asn986-Leu1152
Accession # Q9UIW2
Specificity
Detects human Plexin A1 in direct ELISAs.
In Western blots, no cross-reactivity
with recombinant human Plexin A4, B1, B2, B3, C1, D1, recombinant mouse Plexin A1,
A2, or A3 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Plexin A1 Antibody
Detection of Human Plexin A1 by Western Blot.
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human Plexin A1 Monoclonal Antibody (Catalog # MAB6536) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Plexin A1 at approximately 210 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Plexin A1 in Human Dendritic Cells.
Plexin A1 was detected in immersion fixed immature human dendritic cells using Mouse Anti-Human Plexin A1 Monoclonal Antibody (Catalog # MAB6536) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human Plexin A1 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed immature human dendritic cells
Sample: Immersion fixed immature human dendritic cells
Western Blot
1 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line.
Sample: K562 human chronic myelogenous leukemia cell line.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Plexin A1
Plexin A1 is an approximately 200 kDa type I transmembrane member of the Plexin family of Semaphorin signal transducers. It consists of a 1218 amino acid (aa) extracellular domain with one Sema domain, a spacer, and four tandem IPT/TIG domains, a 21 aa transmembrane segment, and a 631 aa cytoplasmic domain. Plexin A1 binds the transmembrane Semaphorin 6D directly and binds the Class 3 secreted Semaphorins indirectly via Neuropilin-1 and -2. It mediates cytoskeletal remodeling that results in cell migration and axon repulsion. Within aa 964-1152 (in the IPT/TIG region), human Plexin A1 shares 93% aa sequence identity with mouse and rat Plexin A1.
Alternate Names
NOVP, PLXN1, PLXNA1
Gene Symbol
PLXNA1
UniProt
Additional Plexin A1 Products
Product Documents for Human Plexin A1 Antibody
Product Specific Notices for Human Plexin A1 Antibody
For research use only
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