Human PON2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4344
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 453709
Product Specifications
Immunogen
E. coli-derived recombinant human PON2
Ala30-Leu354
Accession # NP_000296
Ala30-Leu354
Accession # NP_000296
Specificity
Detects endogenous human PON2 in Western blots. In Western blots, this antibody does not cross‑react with recombinant human (rh) PON1 or rhPON3.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Human PON2 Antibody
Detection of Human PON2 by Western Blot.
Western blot shows lysates of human liver tissue, Huh-7 human hepatoma cell line, HepG2 human hepatocellular carcinoma cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human PON2 Monoclonal Antibody (Catalog # MAB4344) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for PON2 at approximately 40-42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.Applications for Human PON2 Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: Human liver tissue, Huh-7 human hepatoma cell line, HepG2 human hepatocellular carcinoma cell line, and HeLa human cervical epithelial carcinoma cell line
Sample: Human liver tissue, Huh-7 human hepatoma cell line, HepG2 human hepatocellular carcinoma cell line, and HeLa human cervical epithelial carcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PON2
Long Name
Paraoxonase 2
Alternate Names
A-esterase 2, Serum Aryldialkylphosphatase 2
Gene Symbol
PON2
UniProt
Additional PON2 Products
Product Documents for Human PON2 Antibody
Product Specific Notices for Human PON2 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...