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Human PP14/Glycodelin Antibody

R&D Systems, part of Bio-Techne | Catalog # AF842

R&D Systems, part of Bio-Techne
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AF842
AF842-SP

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Western Blot

Cited:

ELISA Development, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human PP14/Glycodelin
Met19-Phe180 (Glu95Gly)
Accession # P09466

Specificity

Detects human PP14/Glycodelin in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human PP14/Glycodelin Antibody

Detection of Human PP14/Glycodelin antibody by Western Blot.

Detection of Human PP14/Glycodelin by Western Blot.

Western blot shows lysates of human uterus tissue and human placenta tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human PP14/Glycodelin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF842) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PP14/Glycodelin at approximately 24-27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human PP14/Glycodelin Antibody

Application
Recommended Usage

ELISA

This antibody functions as an ELISA detection antibody when paired with Mouse Anti-Human PP14/Glycodelin Monoclonal Antibody(Catalog # MAB842).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Western Blot

0.2 µg/mL
Sample: Human uterus tissue and human placenta tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PP14/Glycodelin

Placenta Protein 14 (PP14, also known as Glycodelin-A) is a 28-kDa glycoprotein that is mainly expressed by late secretory-phase endometrium and first-trimester gestational endometrium. It is present at high levels in amniotic fluid, and in the serum of pregnant women. PP14 has been reported to have immuno- suppressive activity that may play a role in fetal survival during pregnancy.

Long Name

Placental Protein 14

Alternate Names

Glycodelin, PAEP

Entrez Gene IDs

5047 (Human)

Gene Symbol

PAEP

UniProt

Additional PP14/Glycodelin Products

Product Documents for Human PP14/Glycodelin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human PP14/Glycodelin Antibody

For research use only

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