Human PP2C gamma Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5595
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 528322
Product Specifications
Immunogen
E. coli-derived recombinant human PP2C gamma
Met1-Asp546
Accession # O15355
Met1-Asp546
Accession # O15355
Specificity
Detects human PP2C gamma.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human PP2C gamma Antibody
Detection of Human PP2C gamma/PPM1G by Western Blot.
Western blot shows lysates of HUVEC human umbilical vein endothelial cells and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.25 µg/mL of Human PP2C gamma/PPM1G Monoclonal Antibody (Catalog # MAB5595) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PP2C gamma/PPM1G at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.Applications for Human PP2C gamma Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver and human liver cancer tissue
Sample: Immersion fixed paraffin-embedded sections of human liver and human liver cancer tissue
Western Blot
0.25 µg/mL
Sample: HUVEC human umbilical vein endothelial cells and HeLa human cervical epithelial carcinoma cell line
Sample: HUVEC human umbilical vein endothelial cells and HeLa human cervical epithelial carcinoma cell line
Reviewed Applications
Read 1 review rated 5 using MAB5595 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PP2C gamma
Long Name
Protein Phosphatase 2C gamma
Alternate Names
PP2CG, PPM1C, PPM1G, PPP2CG
Gene Symbol
PPM1G
UniProt
Additional PP2C gamma Products
Product Documents for Human PP2C gamma Antibody
Product Specific Notices for Human PP2C gamma Antibody
For research use only
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