Human PRDM14 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6175
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 647002
Product Summary for Human PRDM14 Antibody
Immunogen
E. coli-derived recombinant human PRDM14
Ala2-Asp201
Accession # Q9GZV8
Ala2-Asp201
Accession # Q9GZV8
Specificity
Detects human PRDM14 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse PRDM14, recombinant human (rh) ZNF24, rhZNF143, rhZNF206 or rhZNF281 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human PRDM14 Antibody
Detection of Human PRDM14 by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 2 µg/mL of Human PRDM14 Monoclonal Antibody (Catalog # MAB6175) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PRDM14 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
PRDM14 in BG01V Human Cell Line.
PRDM14 was detected in fixed BG01V human embryonic stem cells grown on irradiated MEF cells using Human PRDM14 Monoclonal Antibody (Catalog # MAB6175) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human PRDM14 Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells grown on iMEFs
Sample: Immersion fixed BG01V human embryonic stem cells grown on iMEFs
Western Blot
2 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line
Sample: HepG2 human hepatocellular carcinoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PRDM14
PRDM14 is an approximately 65 kDa transcriptional inhibitor that contains one histone methylating PR/SET domain (aa 266-367) and six zinc finger repeats (aa 400-568). PRDM14 is preferentially expressed in germ cells and undifferentiated embryonic stem cells in which it plays a central role in lineage specification, maintenance of pluripotency, and repression of somatic gene transcription. PRDM14 is up regulated in breast cancer and lymphoblastic leukemia. Within aa 1-201, human and mouse PRDM14 share 46% aa sequence identity.
Long Name
PR Domain Containing 14
Alternate Names
MGC59730, PFM11, PR domain containing 14, PR domain zinc finger protein 14, PR domain-containing protein 14, PR-domain zinc finger protein 14
Gene Symbol
PRDM14
UniProt
Additional PRDM14 Products
Product Documents for Human PRDM14 Antibody
Product Specific Notices for Human PRDM14 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...