Human/Primate Ig Kappa Light Chain Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB100502
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Human, Primate
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2495C
Product Specifications
Immunogen
Human Ig Kappa Light Chain
Specificity
Detects human Ig Kappa Light Chain in direct ELISAs. Detects human and primate Ig Kappa Light Chain in Western blots.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human/Primate Ig Kappa Light Chain Antibody
Detection of Human and Primate Ig Kappa Light Chain by Western Blot.
Western blot shows human kappa light chain protein, human lambda light chain protein (negative control), human serum, primate serum, mouse serum (negative control), and rat serum (negative control). PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human/Primate Ig Kappa Light Chain Monoclonal Antibody (Catalog # MAB100502) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Ig Kappa Light Chain at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Primate Ig Kappa Light Chain Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: Human kappa light chain protein, Human serum, and Primate serum
Sample: Human kappa light chain protein, Human serum, and Primate serum
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Ig Kappa Light Chain
The immunoglobulin light chain is the smaller subunit of an antibody and in humans can be expressed in two types: as a kappa ( kappa) or a lambda ( lambda) chain. Antibodies are produced by B-Cells that are clonal and each expresses only one type of light chain. The light chain class remains fixed for the life of the B lymphocyte. The ratio of kappa and lambda light chains can be used to determine disease status by immunohistochemistry or ELISA based assays.
Long Name
Immunoglobulin Kappa Light Chain
Alternate Names
ig kappa chain c region;Immunoglobulin K;immunoglobulin kappa light chain;Kappa LC
Additional Ig Kappa Light Chain Products
Product Documents for Human/Primate Ig Kappa Light Chain Antibody
Product Specific Notices for Human/Primate Ig Kappa Light Chain Antibody
For research use only
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