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Human Prostasin/Prss8 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4599

R&D Systems, part of Bio-Techne
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MAB4599
MAB4599-SP

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 530622

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Prostasin/Prss8
Ala33-Gly319
Accession # Q16651

Specificity

Detects human Prostasin/Prss8 in direct ELISAs and Western blots.  In direct ELISAs, less than 15% cross-reactivity with recombinant mouse Prostasin/Prss8 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Prostasin/Prss8 Antibody

Detection of Human Prostasin/Prss8 antibody by Western Blot.

Detection of Human Prostasin/Prss8 by Western Blot.

Western blot shows lysates of human prostate tissue. PVDF Membrane was probed with 2 µg/mL of Human Prostasin/Prss8 Monoclonal Antibody (Catalog # MAB4599) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Prostasin/Prss8 at approximately 40 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Prostasin/Prss8 antibody in Human Prostate by Immunohistochemistry (IHC-P).

Prostasin/Prss8 in Human Prostate.

Prostasin/Prss8 was detected in immersion fixed paraffin-embedded sections of human prostate using Human Prostasin/Prss8 Monoclonal Antibody (Catalog # MAB4599) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Prostasin/Prss8 Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Prostasin/Prss8 (Catalog # 4599-SE), see our available Western blot detection antibodies

Western Blot

2 µg/mL
Sample: Human prostate tissue under non-reducing conditions only

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Prostasin/Prss8

Prostasin, also known as Prss8 and channel activating protease 1, is a 40 kDa serine protease with trypsin-like substrate specificity that is a member of the peptidase S1 family. The enzyme is synthesized from a 343 amino acid (aa) preproenzyme that contains a 29 aa signal peptide, a propeptide (aa 30-32) and a 290 aa mature chain. A second propeptide (aa 323-343) contains a 21 aa transmembrane region (GPI anchor) which can be proteolytically processed to generate a secreted form of the enzyme. The mature chain forms a heterodimer made up of a light chain (aa 33-44) and a heavy chain (aa 45-322) that are connected by disulfide bonds. In addition, aa 45-286 constitute a peptidase S1 domain, and aa 159 is a potential site for N-linked glycosylation. Mature human Prostasin is 79% and 78% aa identical to mature mouse and rat Prostasin, respectively. Prostasin is highly expressed in the prostate gland, and is expressed at lower levels in the lung, kidney, salivary gland, and pancreas. It activates amiloride-sensitive sodium channels.

Alternate Names

Prss8

Entrez Gene IDs

5652 (Human); 76560 (Mouse)

Gene Symbol

PRSS8

UniProt

Additional Prostasin/Prss8 Products

Product Documents for Human Prostasin/Prss8 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Prostasin/Prss8 Antibody

For research use only

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