Human Proteinase 3/Myeloblastin/PRTN3 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6134

R&D Systems, part of Bio-Techne
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MAB6134
MAB6134-SP
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 684042

View all Proteinase 3/Myeloblastin/PRTN3 Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf21-derived recombinant human Proteinase 3/Myeloblastin/PRTN3
Ala26-Arg249
Accession # P24158

Specificity

Detects human Proteinase 3/Myeloblastin/PRTN3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Granzyme B is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human Proteinase 3/Myeloblastin/PRTN3 Antibody

Detection of Human Proteinase 3/Myeloblastin/PRTN3 antibody by Western Blot.

Detection of Human Proteinase 3/Myeloblastin/PRTN3 by Western Blot.

Western blot shows lysates of human granulocytes. PVDF Membrane was probed with 1 µg/mL of Human Proteinase 3/Myeloblastin/PRTN3 Mono-clonal Antibody (Catalog # MAB6134) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Proteinase 3/Myeloblastin/PRTN3 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Proteinase 3/Myeloblastin/PRTN3 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human granulocytes

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Proteinase 3/Myeloblastin/PRTN3

Proteinase 3 (PRTN3), also known as Myeloblastin (MBN), is a 32-33 kDa member of the peptidase S1 family of enzymes. It is expressed by monocytes and neutrophils, the latter of which either secretes it, sequesters it in azurophilic granules, or expresses it on the cell surface. When secreted, it acts on HK and activates the kinin pathway. In azurophilic granules, it aids in the digestion of phagocytosed material. On the cell surface, it likely acts on ECM. Human PRTN3 proprecursor is 231 amino acids (aa) in length. It contains an Ala26Glu27 propeptide that is removed during maturation, a 221 aa mature enzyme (aa 28-248), and an eight aa C‑terminal propeptide (aa 249-256). Within the cell, a 35 kDa immature form exists; on the cell surface, both constitutively inactive, and induced active forms may be found, often in a noncovalent association with CD177/NB1. Over aa 26-249, human PRTN3 shares 68% aa identity with mouse PRTN3.

Alternate Names

ACPA, AGP7, C-ANCA, MBN, MBT, Myeloblastin, NP4, PRTN3

Entrez Gene IDs

5657 (Human); 19152 (Mouse); 314615 (Rat)

Gene Symbol

PRTN3

UniProt

P24158 (Human)

Additional Proteinase 3/Myeloblastin/PRTN3 Products

Product Documents for Human Proteinase 3/Myeloblastin/PRTN3 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human Proteinase 3/Myeloblastin/PRTN3 Antibody

For research use only

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