Human Protocadherin-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5899
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Protocadherin‑1
Thr58-Asn162
Accession # Q08174
Thr58-Asn162
Accession # Q08174
Specificity
Detects human Protocadherin-1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) Protocadherin-8 and rhProtocadherin-10 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Protocadherin-1 Antibody
Detection of Human Protocadherin-1 by Western Blot.
Western blot shows lysates of human hippocampus tissue. PVDF membrane was probed with 1 µg/mL of Human Protocadherin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5899) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Protocadherin-1 at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Protocadherin-1 in BG01V Human Stem Cells.
Protocadherin-1 was detected in immersion fixed BG01V human embryonic stem cells using Human Protocadherin-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5899) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human Protocadherin-1 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed BG01V human embryonic stem cells
Sample: Immersion fixed BG01V human embryonic stem cells
Western Blot
1 µg/mL
Sample: Human hippocampus tissue
Sample: Human hippocampus tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Protocadherin-1
Alternate Names
PC42, PCDH1, PCDH42, Protocadherin1
Gene Symbol
PCDH1
UniProt
Additional Protocadherin-1 Products
Product Documents for Human Protocadherin-1 Antibody
Product Specific Notices for Human Protocadherin-1 Antibody
For research use only
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