Human PSMA/FOLH1/NAALADase I APC-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB4234A
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)
Antibody Source
Monoclonal Mouse IgG2A Clone # 460420
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human PSMA/FOLH1/NAALADase I
Lys44-Ala750
Accession # Q04609
Lys44-Ala750
Accession # Q04609
Specificity
Detects human PSMA/FOLH1/NAALADase I in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) NAALADase-like 2 and no cross-reactivity with rhNAALADase-like 1, rhNAALADase-like 3, recombinant mouse (rm) PSMA/FOLH1/NAALADase I, or rmNAALAase-like 2 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human PSMA/FOLH1/NAALADase I APC-conjugated Antibody
Detection of PSMA/FOLH1/NAALADase I in LNCaP Human Cell Line by Flow Cytometry.
LNCaP human prostate cancer cell line was stained with Mouse Anti-Human PSMA/FOLH1/NAALADase I APC-conjugated Monoclonal Antibody (Catalog # FAB4234A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.Applications for Human PSMA/FOLH1/NAALADase I APC-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: LNCaP human prostate cancer cell line
Sample: LNCaP human prostate cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: PSMA/FOLH1/NAALADase I
Human prostate-specific membrane antigen (PSMA), a tumor marker in prostate cancer encoded by the FOLH1 gene, is a type II transmembrane zinc metallopeptidase that is most highly expressed in the nervous system, prostate, kidney, and small intestine (1, 2). The enzyme is also known as glutamate carboxypeptidase II (GCPII), folate hydrolase 1, folypoly-gamma-glutamate carboxypeptidase (FGCP), and N-acetylated-alpha-linked acidic dipeptidase I (NAALADase I). In the brain, PSMA hydrolyzes the neurotransmitter N-acetyl-Asp-Glu to produce glutamate, another neurotransmitter. Inhibition of brain PSMA activity is considered to be a promising approach for the treatment of neurological disorders associated with glutamate excitotoxicity, such as stroke, chronic pain, and amyotrophic lateral sclerosis (3). Intestinal PSMA hydrolyzes folylpoly-gamma -glutamates, facilitating the uptake of folate (4).
References
- Silver, D.A. et al. (1997) Clin. Cancer Res. 3:81.
- Carter, R.E. et al. (1996) Pro. Natl. Acad. Sci. USA. 93:749.
- Jackson, P.F. and Slusher, B.S. (2001) Curr. Med. Chem. 8:949.
- Heston, W.D. (1997) Urology 49:104.
Long Name
Prostate-specific Membrane Antigen
Alternate Names
FGCP, FOLH1, GCP2, GCPII, mopsm, NAALAD1, NAALADase I
Gene Symbol
FOLH1
UniProt
Additional PSMA/FOLH1/NAALADase I Products
Product Documents for Human PSMA/FOLH1/NAALADase I APC-conjugated Antibody
Product Specific Notices for Human PSMA/FOLH1/NAALADase I APC-conjugated Antibody
For research use only
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