Detects human PSMA/FOLH1/NAALADase I in direct ELISA and Western blot. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) NAALADase-like 2 and no cross-reactivity with rhNAALADase-like 1, rhNAALADase-like 3, recombinant mouse (rm) NAALADase I, or rmNAALAase-like 2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Scientific Data Images for Human PSMA/FOLH1/NAALADase I Antibody

Detection of Human NAALADase I by Western Blot.

Western blot shows lysates of human prostate tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human PSMA/FOLH1/NAALADase I Monoclonal Antibody (Catalog # MAB4234) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NAALADase I at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of NAALADase I in LNCaP Human Cell Line by Flow Cytometry.

LNCaP human prostate cancer cell line was stained with Mouse Anti-Human PSMA/FOLH1/NAALADase I Monoclonal Antibody (Catalog # MAB4234, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).

NAALADase I in PC‑3 Human Cell Line.

NAALADase I was detected in immersion fixed PC-3 human prostate cancer cell line using Mouse Anti-Human PSMA/FOLH1/ NAALADase I Monoclonal Antibody (Catalog # MAB4234) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

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Applications for Human PSMA/FOLH1/NAALADase I Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/10⁶ cells
Sample: LNCaP human prostate cancer cell line

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed PC‑3 human prostate cancer cell line

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human prostate

Western Blot

2 µg/mL
Sample: Human prostate tissue

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using MAB4234 in the following applications:

ELISA (1 Review)
Simple Western (1 Review)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PSMA/FOLH1/NAALADase I

Human prostate-specific membrane antigen (PSMA), a tumor marker in prostate cancer encoded by the FOLH1 gene, is a type II transmembrane zinc metallopeptidase that is most highly expressed in the nervous system, prostate, kidney, and small intestine (1, 2). The enzyme is also known as glutamate carboxypeptidase II (GCPII), folate hydrolase 1, folypoly-gamma-glutamate carboxypeptidase (FGCP), and N-acetylated-alpha-linked acidic dipeptidase I (NAALADase I). In the brain, PSMA hydrolyzes the neurotransmitter N-acetyl-Asp-Glu to produce glutamate, another neurotransmitter. Inhibition of brain PSMA activity is considered to be a promising approach for the treatment of neurological disorders associated with glutamate excitotoxicity, such as stroke, chronic pain, and amyotrophic lateral sclerosis (3). Intestinal PSMA hydrolyzes folylpoly-gamma -glutamates, facilitating the uptake of folate (4).

References

Silver, D.A. et al. (1997) Clin. Cancer Res. 3:81.
Carter, R.E. et al. (1996) Pro. Natl. Acad. Sci. USA. 93:749.
Jackson, P.F. and Slusher, B.S. (2001) Curr. Med. Chem. 8:949.
Heston, W.D. (1997) Urology 49:104.

Long Name

Prostate-specific Membrane Antigen

Alternate Names

FGCP, FOLH1, GCP2, GCPII, mopsm, NAALAD1, NAALADase I

Entrez Gene IDs

2346 (Human); 53320 (Mouse); 85309 (Rat); 101866140 (Cynomolgus Monkey)

Gene Symbol

FOLH1

UniProt

Q04609

Additional PSMA/FOLH1/NAALADase I Products

Product Documents for Human PSMA/FOLH1/NAALADase I Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human PSMA/FOLH1/NAALADase I Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human PSMA/FOLH1/NAALADase I Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4234

Key Product Details

Species Reactivity

Validated:

Cited:

Applications