Human PTGER2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6656
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 607077
Product Specifications
Immunogen
NS0 mouse myeloma cell line transfected with human PTGER2
Accession # P43116
Accession # P43116
Specificity
Detects human PTGER2 in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human PTGER2 Antibody
PTGER2 in Human Bladder.
PTGER2 was detected in immersion fixed paraffin-embedded sections of human bladder using Human PTGER2 Monoclonal Antibody (Catalog # MAB6656) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Strongest staining was localized to umbrella cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human PTGER2 by Western Blot.
Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF Membrane was probed with 2 µg/mL of Human PTGER2 Monoclonal Antibody (Catalog # MAB6656) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PTGER2 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human PTGER2 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human bladder
Sample: Immersion fixed paraffin-embedded sections of human bladder
Western Blot
2 µg/mL
Sample: MCF‑7 human breast cancer cell line
Sample: MCF‑7 human breast cancer cell line
Reviewed Applications
Read 1 review rated 4 using MAB6656 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PTGER2
Long Name
Prostaglandin E2 Receptor 2, Subtype EP2
Alternate Names
EP2
Gene Symbol
PTGER2
UniProt
Additional PTGER2 Products
Product Documents for Human PTGER2 Antibody
Product Specific Notices for Human PTGER2 Antibody
For research use only
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