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Human RAMP1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF6428

R&D Systems, part of Bio-Techne
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AF6428
AF6428-SP

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

Product Specifications

Immunogen

E. coli-derived recombinant human RAMP1
Cys27-Ser117
Accession # O60894

Specificity

Detects human RAMP1 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse RAMP1 is observed and less than 1% cross-reactivity with recombinant human (rh) RAMP2 and rhRAMP3 is observed.  

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human RAMP1 Antibody

Detection of Human RAMP1 antibody by Western Blot.

Detection of Human RAMP1 by Western Blot.

Western blot shows lysates of human heart tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human RAMP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6428) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for RAMP1 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
RAMP1 antibody in Human Brain by Immunohistochemistry (IHC-P).

RAMP1 in Human Brain.

RAMP1 was detected in immersion fixed paraffin-embedded sections of human brain (hypothalamus) using Sheep Anti-Human RAMP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6428) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal and glial processes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

RAMP1 in Human Thyroid Cancer Tissue..

RAMP1 was detected in immersion fixed paraffin-embedded sections of human thyroid cancer tissue using Sheep Anti-Human RAMP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6428) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (VC006). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to vasculature. Staining was performed using our protocol forIHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human RAMP1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (hypothalamus) and immersion fixed paraffin-embedded sections of human thyroid cancer tissue

Western Blot

1 µg/mL
Sample: Human heart tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RAMP1

RAMP1 (Receptor Activity Modifying Protein 1) is a 14‑18 kDa member of the RAMP family of proteins. Variability in MW is due to the absence, or presence, of intramolecular disulfide bonds that form in the ER and Golgi. It is expressed on/in neurons, vascular endothelial cells, visceral and vascular smooth muscle cells, mammary epithelium, osteoblasts and cardiomyocytes. RAMP1 interacts with CRLR/CLR to form an 84 kDa noncovalent receptor complex for CGRP and AM, and with CTR to form a 76 kDa receptor complex for amylin. Mature human RAMP1 is a 122 amino acid (aa) nonglycosylated type I transmembrane protein that contains a 91 aa extracellular domain (ECD) (aa 27‑117) plus a ten aa cytoplasmic tail. In the ECD, residues 78‑90 bind AM, while residues 91‑103 bind CGRP. In the absence of CRLR and CTR, RAMP1 will form 30‑32 kDa disulfide‑linked homodimers in the ER/Golgi. Over aa 27‑117, human RAMP1 shares 65% aa identity with mouse RAMP1.

Long Name

Receptor Activity Modifying Protein 1

Alternate Names

calcitonin receptor-like receptor activity modifying protein 1, Calcitonin-receptor-like receptor activity-modifying protein 1, CRLR activity-modifying protein 1, receptor (calcitonin) activity modifying protein 1, receptor (G protein-coupled) activity modifying protein 1, receptor activity modifying protein 1, receptor activity-modifying protein 1

Entrez Gene IDs

10267 (Human); 51801 (Mouse); 58965 (Rat)

Gene Symbol

RAMP1

UniProt

Additional RAMP1 Products

Product Documents for Human RAMP1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human RAMP1 Antibody

For research use only

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