Human/Rat Tollip Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4678
Key Product Details
Species Reactivity
Validated:
Human, Rat
Cited:
Fish - Danio rerio (Zebrafish)
Applications
Validated:
Western Blot
Cited:
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 503315
Product Specifications
Immunogen
E. coli-derived recombinant human Tollip
Met1-Pro274
Accession # Q9H0E2
Met1-Pro274
Accession # Q9H0E2
Specificity
Detects human and rat Tollip in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human/Rat Tollip Antibody
Detection of Human/Rat Tollip by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and NRK rat normal kidney cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Rat Tollip Monoclonal Antibody (Catalog # MAB4678) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Tollip at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.Applications for Human/Rat Tollip Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and NRK rat normal kidney cells
Sample: HepG2 human hepatocellular carcinoma cell line and NRK rat normal kidney cells
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Tollip
Tollip (Toll-interacting protein) is a 28 kDa, cytoplasmic protein that is involved in IL-1 and Toll receptor signaling. It is classified as an adaptor protein that performs multiple functions. It binds both IRAK and Toll receptors, inhibiting constitutive IL-1 and Toll receptor signaling. It also binds to ubiquinated IL-1 RI, directing it to the endosomal degradation compartment. Human Tollip is 274 amino acids(aa) in length. It contains a C2 Ca++-binding region (aa 54‑154) and a CUE domain (aa 229‑270) that binds ubiquitin. One potential splice form exists that shows a deletion of aa 6‑33. Human Tollip is 92% identical to mouse Tollip.
Long Name
Toll Interacting Protein
Alternate Names
IL-1RAcPIP
Gene Symbol
TOLLIP
UniProt
Additional Tollip Products
Product Documents for Human/Rat Tollip Antibody
Product Specific Notices for Human/Rat Tollip Antibody
For research use only
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