Detects human RBP4/Retinol‑Binding Protein 4 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 25% cross-reactivity with recombinant mouse RBP4 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human RBP4/Retinol-Binding Protein 4 Antibody

Detection of RBP4/Retinol-Binding Protein 4 in HepG2 Human Cell Line by Flow Cytometry.

HepG2 human hepato-cellular carcinoma cell line was stained with Mouse Anti-Human RBP4/Retinol-Binding Protein 4 Monoclonal Antibody (Catalog # MAB3378, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.

Applications for Human RBP4/Retinol-Binding Protein 4 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells
Sample: HepG2 human hepatocellular carcinoma cell line fixed with paraformaldehyde and permeabilized with methanol

Western Blot

1 µg/mL
Sample: Recombinant Human RBP4/Retinol-Binding Protein 4 (Catalog # 3378-LC)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RBP4/Retinol-Binding Protein 4

Retinol (also known as vitamin A) is unstable and insoluble in the aqueous solution (1, 2). However, retinol becomes quite stable and soluble in plasma due to its tight interaction with retinol-binding protein 4 (RBP4), also known as plasma retinol-binding protein. A prototypic member of the lipocalin superfamily, RBP4 has a beta-barrel structure with a well-defined cavity. It is secreted from the liver, a process requiring the availability of retinol. RBP4 delivers retinol from the liver to the peripheral tissues. In plasma, the RBP4-retinol complex interacts with transthyretin (TTR), also known as thyroxine-binding protein and prealbumin. The retinol-RBP4-TTR complex prevents the loss of RBP4 by filtration through the kidney and increases the stability of the retinol-RBP4 complex. Defects in RBP4 cause retinol-binding protein deficiency, which affects night vision.

References

Zanotti, G. and R. Berni. (2004) Vitamins and Hormones 69:271.
Newcomer, M.E. and D.E. Ong. (2000) Biochim. Biophys. Acta 1482:57.

Alternate Names

RetinolBinding Protein 4

Entrez Gene IDs

5950 (Human); 19662 (Mouse); 25703 (Rat)

Gene Symbol

RBP4

UniProt

P02753

Additional RBP4/Retinol-Binding Protein 4 Products

Product Documents for Human RBP4/Retinol-Binding Protein 4 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human RBP4/Retinol-Binding Protein 4 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human RBP4/Retinol-Binding Protein 4 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB3378

Key Product Details

Species Reactivity

Validated:

Cited:

Applications