Human RCOR1/CoREST Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6047
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 658526
Product Specifications
Immunogen
E. coli-derived recombinant human RCOR1/CoREST
Ala394-Ser482
Accession # Q9UKL0
Ala394-Ser482
Accession # Q9UKL0
Specificity
Detects human RCOR1/CoREST in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human RCOR3 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human RCOR1/CoREST Antibody
Detection of Human RCOR1/CoREST by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line and MOLT-4 human acute lymphoblastic leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Human RCOR1/CoREST Monoclonal Antibody (Catalog # MAB6047) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for RCOR1/CoREST at approximately 64-66 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
RCOR1/CoREST in HeLa Human Cell Line.
RCOR1/CoREST was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Human RCOR1/CoREST Monoclonal Antibody (Catalog # MAB6047) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human RCOR1/CoREST Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Western Blot
1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and MOLT‑4 human acute lymphoblastic leukemia cell line
Sample: Jurkat human acute T cell leukemia cell line and MOLT‑4 human acute lymphoblastic leukemia cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RCOR1/CoREST
RCOR1 (REST [RE-1 silencing transcription factor] CORepressor 1; also CoREST) is a 64-66 kDa member of the CoREST family of proteins. It is a nuclear protein that is ubiqui-tously expressed. RCOR1 serves as a molecular bridge, linking REST with Sin3, HDAC, LSD1, histone H3 K9 me-thyltransferases, and MeCP2. This creates a complex that represses the expression of neuron-specific genes in nonneuronal cells, principally by coordinating the demethylation of Lys4 in histone H3. Human RCOR1 is 482 amino acids (aa) in length. It contains one ELM2 domain (aa 102-157) that interacts with HDAC, and two SANT sequences (aa 187-238 and 378‑429) that bind DNA tandem repeats. There are three potential RCOR1 splice vari-ants. One contains a four aa substitution for aa 296-482, a second shows an alternative start site 3 aa upstream of the standard site, while a third shares the same upstream start site accompanied by a 15 aa substitution for aa 471-482. Over aa 394-482, human RCOR1 shares 93% aa identity with mouse RCOR1.
Long Name
REST Corepressor 1
Alternate Names
CoREST, RCOR1
Entrez Gene IDs
23186 (Human)
Gene Symbol
RCOR1
UniProt
Additional RCOR1/CoREST Products
Product Documents for Human RCOR1/CoREST Antibody
Product Specific Notices for Human RCOR1/CoREST Antibody
For research use only
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