Human RIPK2/RIP2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB103872
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2527A
Product Specifications
Immunogen
E.coli-derived recombinant human RIPK2/RIP2
Met1-Met540
Accession # O43353
Met1-Met540
Accession # O43353
Specificity
Detects human RIPK2/RIP2 in direct ELISAs.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human RIPK2/RIP2 Antibody
Detection of Human RIPK2/RIP2 by Western Blot.
Western blot shows lysates of Ramos human Burkitt's lymphoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human RIPK2/RIP2 Monoclonal Antibody (Catalog # MAB103872) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for RIPK2/RIP2 at approximately 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.Applications for Human RIPK2/RIP2 Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: Ramos human Burkitt's lymphoma cell line and HeLa human cervical epithelial carcinoma cell line
Sample: Ramos human Burkitt's lymphoma cell line and HeLa human cervical epithelial carcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RIPK2/RIP2
Receptor-interacting serine/threonine-protein kinase 2, also known as RIPK2 or RIP-2, is a 540 amino acids (aa) Serine/threonine/tyrosine kinase encoded by the RIPK2 gene. RIP-2, a member of the receptor-interacting protein (RIP) family of serine/threonine protein kinases, plays an essential role in modulation of innate and adaptive immune responses. Human RIP2 is an adapter protein necessary for signal propagation of the Nucleotide-binding-oligomerization-domain-containing proteins 1/2 (NOD1 and NOD2). Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize, and recruit RIPK2 through the C-terminal caspase recruitment domain (CARD). Human and mouse RIPK2 aa sequences are 84% identical .
Long Name
Receptor Interacting Serine/Threonine Kinase 2
Alternate Names
CARD3, GIG30, RICK, RIP-2, RIP2
Gene Symbol
RIPK2
UniProt
Additional RIPK2/RIP2 Products
Product Documents for Human RIPK2/RIP2 Antibody
Product Specific Notices for Human RIPK2/RIP2 Antibody
For research use only
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