Human Rit2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6094
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 456313
Product Specifications
Immunogen
E. coli-derived recombinant human Rit2
aa 1-217
Accession # Q99578
aa 1-217
Accession # Q99578
Specificity
Detects human Rit2 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Rit2 Antibody
Detection of Human Rit2 by Western Blot.
Western blot shows lysates of human brain (cerebellum) tissue and human brain (hypothalamus) tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human Rit2 Monoclonal Antibody (Catalog # MAB6094) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Rit2 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human Rit2 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion-fixed paraffin-embedded sections of human brain (caudate putamen)
Sample: Immersion-fixed paraffin-embedded sections of human brain (caudate putamen)
Western Blot
2 µg/mL
Sample: Human brain (cerebellum) tissue and human brain (hypothalamus) tissue
Sample: Human brain (cerebellum) tissue and human brain (hypothalamus) tissue
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Rit2
GTP-binding protein Rit2 (Rit2; also Rin, Ras-like protein expressed in neurons, Ras-like without CAAX) is a 25 kDa member of the small GTPase superfamily and Ras family. Human Rit2 is 217 amino acids (aa) in length. Like all small G-proteins, Rit2 contains five highly conserved domains (G1-G5) and acts as a molecular switch by alternating an active GTP-bound form and an inactive GDP-bound form. There are two isoforms for Rit2. Isoform 1 is the long form. Isoform 2 has an 11 aa substitution for residues 143‑153 found in isoform 1 and a deletion of aa 154‑217. Rit2 is expressed exclusively in neurons. In addition, Rit2 binds calmodulin through a C‑terminal binding motif.
Long Name
Ras-like without CAAX 2
Alternate Names
RIBA, RIN, ROC2
Gene Symbol
RIT2
UniProt
Additional Rit2 Products
Product Documents for Human Rit2 Antibody
Product Specific Notices for Human Rit2 Antibody
For research use only
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