Human ROBO1 Antibody

Detection of Human ROBO1 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and SH-SY5Y human neuroblastoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ROBO1 Monoclonal Antibody (Catalog # MAB7118) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ROBO1 at approximately 220 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human ROBO1 by Western Blot

NRP1, ROBO1, and ITG alpha5 were identified as target genes of miR-148a/b-3p.a Venn diagrams illustrate the intersection between three algorithms that were used to predict potential gene targets of miR-148a/b-3p. b RT-PCR analysis indicates that the expression of NRP1, ROBO1, and ITG alpha5 increased during UCB-MNC to OEC differentiation. c The mRNA levels of NRP1, ROBO1, and ITGɑ5 in ECs were regulated by miR-148a/b. ECs were transfected with miR mimics (80 µM), and 48 h after transfection, endogenous NRP1 expression was detected by RT-PCR. d At 24, 48, or 72 h after transfection, Western blotting analysis was performed to investigate the protein levels of target genes. The internal loading control was beta-actin. e Relative mRNA expression of NRP1, ROBO1, and ITGɑ5 was confirmed by RT-PCR. PECAM1 expression was unchanged following treatment with the miR-148a/b-3p mimic (n = 5 per group). f Quantification of the luciferase reporter assay data comparing the wildtype (Wt) NRP1-3′UTR and a mutated (Mut) NRP1-3′UTR; the (Mut) contained a 7-nucleotide mutation in the predicted miR-148a/b-3p binding site. The schematic depicts the predicted miR-148a/b-3p binding site (blue) in the NRP1-3′UTR and shows the 7-nucleotide mutation. All data are presented as the mean ± SEM, **P < 0.01, ***P < 0.001 Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31723119), licensed under a CC-BY license. Not internally tested by R&D Systems.