MDA-MB-231 human breast cancer cell line was stained with Mouse Anti-Human ROR1 Monoclonal Antibody (Catalog # MAB20001, filled histogram) or Mouse IgG1 Isotype Control Antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Applications for Human ROR1 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: MDA-MB-231 human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ROR1

ROR1 belongs to the ROR family of receptor tyrosine kinases, which are characterized by: the extracellular immunoglobulin domain, cysteine-rich domain that resembles the Wnt-binding sites of Frizzled proteins, kringle domain and intracellular kinase domain. Human ROR1 shares 97% and 58% amino acid sequence identity with mouse ROR1 and human ROR2, respectively.

Long Name

Receptor Tyrosine Kinase-like Orphan Receptor 1

Alternate Names

NTRKR1

Entrez Gene IDs

4919 (Human); 26563 (Mouse)

Gene Symbol

ROR1

UniProt

Q01973

Additional ROR1 Products

Product Documents for Human ROR1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human ROR1 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human ROR1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB20001

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all ROR1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human ROR1 Antibody

Detection of ROR1 in MDA‑MB‑231 Human Cell Line by Flow Cytometry.