Human S100A11 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4874
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human S100A11
Ala2-Thr105
Accession # P31949
Ala2-Thr105
Accession # P31949
Specificity
Detects human S100A11 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) S100A1, A2, A7, A8, A10, A13, A16, rhS100B, rhS100P, recombinant mouse (rm) S100A6, and rmS100A10 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human S100A11 Antibody
Detection of Human S100A11 by Western Blot.
Western blot shows lysates of JEG-3 human epithelial choriocarcinoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human S100A11 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4874) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for S100A11 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Human S100A11 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skin
Sample: Immersion fixed paraffin-embedded sections of human skin
Western Blot
1 µg/mL
Sample: JEG-3 human epithelial choriocarcinoma cell line and HeLa human cervical epithelial carcinoma cell line
Sample: JEG-3 human epithelial choriocarcinoma cell line and HeLa human cervical epithelial carcinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: S100A11
S100A11 (also S100C and calgizzarin) is a 10‑12 kDa member of the S100 family, EF-hand superfamily of Ca‑binding proteins. It is produced by smooth muscle and keratinocytes. Intracellularly, it suppresses growth; extracellularly, it exists as both a monomer, homodimer, and heterodimer with S100B, binds to RAGE, induces EGF, and promotes cell growth. Human S100A11 is 105 amino acids (aa) in length. It contains two EF-hand motifs (aa 13‑49 and 55‑90) and one high-affinity Ca‑binding site (aa 68‑79), and binds annexin I with its C-terminal half. There may be one alternative splice form that apparently contains a series of mutations over aa 61‑79 and shows 90% overall aa identity to the standard form. Full-length human S100A11 shares 78% and 82% aa identity with mouse and porcine S100A11, respectively.
Long Name
S100 Calcium Binding Protein A11
Alternate Names
Calgizzarin, MLN70, S100C
Gene Symbol
S100A11
UniProt
Additional S100A11 Products
Product Documents for Human S100A11 Antibody
Product Specific Notices for Human S100A11 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...