Human S1P1/EDG-1 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB2016A

Clone 218713 was used by HLDA to establish CD designation
R&D Systems, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Allophycocyanin (Excitation = 620-650 nm, Emission = 660-670 nm)

Antibody Source

Monoclonal Mouse IgG2B Clone # 218713

View all S1P1/EDG-1 Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human S1P1/EDG‑1
Met1-Ser382
Accession # P21453

Specificity

Detects human S1P1/EDG‑1 by flow cytometry.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human S1P1/EDG-1 APC-conjugated Antibody

Detection of S1P1/EDG-1 antibody in CHO Chinese Hamster Cell Line Transfected with Human S1P1/EDG-1 and eGFP antibody by Flow Cytometry.

Detection of S1P1/EDG‑1 in CHO Chinese Hamster Cell Line Transfected with Human S1P1/EDG-1 and eGFP by Flow Cytometry.

CHO Chinese hamster ovary cell line transfected with either (A) human S1P1/EDG-1 or (B) irrelevant transfectants and eGFP were stained with Mouse Anti-Human S1P1/EDG-1 APC-conjugated Monoclonal Antibody (Catalog # FAB2016A). Quadrant markers were set based on control antibody staining (Catalog # IC0041A). View our protocol for Staining Membrane-associated Proteins.

Applications for Human S1P1/EDG-1 APC-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: CHO Chinese hamster ovary cell line transfected with S1P1/EDG-1
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: S1P1/EDG-1

S1P1, also known as EDG-1, is a member of the endothelial differentiation gene family. It is a high affinity G protein-coupled receptor for the bioactive lipid, Sphingosine-1-phosphate. S1P1 signaling regulates endothelial cell survival, cytoskeletal remodeling, chemotaxis, and angiogenesis.

Long Name

Endothelial Differentiation Gene 1

Alternate Names

CD363, EDG1, S1PR1

Entrez Gene IDs

1901 (Human); 13609 (Mouse)

Gene Symbol

S1PR1

UniProt

P21453

Additional S1P1/EDG-1 Products

Product Documents for Human S1P1/EDG-1 APC-conjugated Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human S1P1/EDG-1 APC-conjugated Antibody

For research use only

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