CHO Chinese hamster ovary cell line transfected with either (A) human S1P₁/EDG-1 or (B) irrelevant transfectants and eGFP were stained with Mouse Anti-Human S1P₁/EDG-1 PE-conjugated Monoclonal Antibody (Catalog # FAB2016P). Quadrant markers were set based on control antibody staining (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.

Applications for Human S1P1/EDG-1 PE-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: CHO Chinese hamster ovary cell line transfected with S1P₁/EDG-1 and eGFP

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: S1P1/EDG-1

S1P₁, also known as EDG-1, is a member of the endothelial differentiation gene family. It is a high affinity G protein-coupled receptor for the bioactive lipid, Sphingosine-1-phosphate. S1P₁ signaling regulates endothelial cell survival, cytoskeletal remodeling, chemotaxis, and angiogenesis.

Long Name

Endothelial Differentiation Gene 1

Alternate Names

CD363, EDG1, S1PR1

Entrez Gene IDs

1901 (Human); 13609 (Mouse)

Gene Symbol

S1PR1

UniProt

P21453

Additional S1P1/EDG-1 Products

Product Documents for Human S1P1/EDG-1 PE-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human S1P1/EDG-1 PE-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human S1P1/EDG-1 PE-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB2016P

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

View all S1P1/EDG-1 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human S1P1/EDG-1 PE-conjugated Antibody

Detection of S1P1/EDG‑1 in CHO Chinese Hamster Cell Line Transfected with Human S1P1/EDG-1 and eGFP by Flow Cytometry.