Human SCL/Tal1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3360
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human SCL
Arg199-Gly266
Accession # P17542
Arg199-Gly266
Accession # P17542
Specificity
Detects human SCL in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human SCL/Tal1 Antibody
Detection of Human SCL/Tal1 by Western Blot.
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human SCL/Tal1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3360) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for SCL/Tal1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for Human SCL/Tal1 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells
Western Blot
1 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line
Sample: K562 human chronic myelogenous leukemia cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SCL/Tal1
Long Name
Stem Cell Leukemia/T-cell Acute Lymphocytic Leukemia 1
Alternate Names
Stem Cell Protein, Tal1, TCL5
Entrez Gene IDs
6886 (Human)
Gene Symbol
TAL1
UniProt
Additional SCL/Tal1 Products
Product Documents for Human SCL/Tal1 Antibody
Product Specific Notices for Human SCL/Tal1 Antibody
For research use only
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