Human Semaphorin 7A Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2068
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
CyTOF-ready, Flow Cytometry, Western Blot
Cited:
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Semaphorin 7A
His47-Ala648
Accession # O75326
His47-Ala648
Accession # O75326
Specificity
Detects human Semaphorin 7A in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse Semaphorin 7A is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human Semaphorin 7A Antibody
Detection of Semaphorin 7A in PBMC lymphocytes treated +/- 5 µg/mL PHA for 2 days by Flow Cytometry
PBMC lymphocytes treated with 5 µg/mL PHA for 2 days (A) vs naïve PBMC lymphocytes (B) were stained with Goat Anti-Human Semaphorin 7A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2068) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107) and Mouse anti-Human CD3 APC-conjugated Monoclonal antibody (Catalog # FAB100A). View our protocol for Staining Membrane-associated Proteins.
Detection of Semaphorin 7A in HT1080 cells by Flow Cytometry
HT1080 cells were stained with Goat Anti-Human Semaphorin 7A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2068, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram) followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.Applications for Human Semaphorin 7A Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: Human T cells treated with PHA (see details below); HT1080 human fibrosarcoma cell line
Sample: Human T cells treated with PHA (see details below); HT1080 human fibrosarcoma cell line
Western Blot
0.1 µg/mL
Sample: Recombinant Human Semaphorin 7A
Sample: Recombinant Human Semaphorin 7A
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Semaphorin 7A
Semaphorin 7A (Sema 7A), also known as Sema L, Sema K1 and CD108, is an 80 kDa glycosylphosphatidylinositol-anchored protein belonging to the Semaphorin superfamily. It binds to the beta1 integrin subunit as well as Plexin C1. The Sema domain of human Sema 7A shares 92% and 90% amino acid sequence homology with that of the mouse and canine protein.
Alternate Names
CD108, H-Sema-L, Sema7A, SEMAL
Gene Symbol
SEMA7A
UniProt
Additional Semaphorin 7A Products
Product Documents for Human Semaphorin 7A Antibody
Product Specific Notices for Human Semaphorin 7A Antibody
For research use only
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