Human Serpin C1/Antithrombin-III Antibody
R&D Systems, part of Bio-Techne | Catalog # AF1267
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunoprecipitation, Neutralization, Western Blot
Cited:
ELISA Capture, Flow Cytometry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Serpin C1/Antithrombin-III
His33-Lys464
Accession # P01008
His33-Lys464
Accession # P01008
Specificity
Detects human Serpin C1/Antithrombin-III in direct ELISAs and Western blots. In direct ELISAs, approximately 45% cross-reactivity with recombinant mouse (rm) Serpin C1 is observed and less than 2% cross‑reactivity with recombinant human (rh) Serpin A1, rhSerpin A3, rhSerpin A4, rhSerpin A5, rhSerpin F2, rmSerpin D1 and rhPEDF is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Applications for Human Serpin C1/Antithrombin-III Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Serpin C1/
Antithrombin-III (Catalog # 1267-PI), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Serpin C1/
Antithrombin-III (Catalog # 1267-PI), see our available Western blot detection antibodies
Western Blot
0.1 µg/mL
Sample: Recombinant Human Serpin C1/Antithrombin-III (Catalog # 1267-PI)
Sample: Recombinant Human Serpin C1/Antithrombin-III (Catalog # 1267-PI)
Neutralization
Measured by its ability to neutralize Recombinant Human Serpin C1 (0.8 µg/mL, Catalog # 1267-PI) inhibition of Recombinant Human Coagulation Factor II/Thrombin (0.05 µg/mL, Catalog # 1473-SE) cleavage of the fluorogenic peptide substrate Boc-VPR-AMC (100 µM, Catalog # ES011). The Neutralization Dose (ND50) is typically 4.6 µg/mL.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using AF1267 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Serpin C1/Antithrombin-III
Serpin C1 is a member of the Serpin superfamily of the serine protease inhibitors (1). It is the principal plasma Serpin of blood clotting proteases and inhibits thrombin as well as several factors such as Xa (2). Similar to Serpins A5 and D1, its thrombin inhibitory activity is enhanced by heparin. Hereditary and acquired Serpin C1 deficiency is the cause of an increased thrombotic tendency in many cases (3). For example, acquired Serpin C1 deficiency is a common condition in sepsis, after major trauma or surgery (4).
References
- Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
- Chuang, Y.-J. et al. (2001) Biochemistry 40:6670.
- Vinazzer, H. (1999) Semin. Thromb. Hemost. 25:257.
- Risberg, B. (1998) Blood Coagul. Fibrinolysis Suppl. 3:S3.
Alternate Names
Antithrombin-III, ATIII
Gene Symbol
SERPINC1
UniProt
Additional Serpin C1/Antithrombin-III Products
Product Documents for Human Serpin C1/Antithrombin-III Antibody
Product Specific Notices for Human Serpin C1/Antithrombin-III Antibody
For research use only
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