HEK293 human embryonic kidney cell line transfected with either (A) human Siglec-15 or (B) irrelevant protein, and eGFP, was stained with Mouse anti-human Siglec-15 monoclonal antibody (Catalog # MAB9227) followed by Allophycocyanin-conjugated anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on control antibody staining (MAB002, data not shown). Staining was performed using our Staining Membrane-Associated Proteins protocol.

Applications for Human Siglec-15 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HEK293 Human Cell Line transfected with Human Siglec-15 and eGFP

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Siglec-15

Siglec-15 is a transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules (1). Mature human Siglec-15 consists of a 244 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain (2). Within the ECD, human Siglec-15 shares 85% aa sequence identity with mouse and rat Siglec-15. Alternative splicing generates an additional isoform that lacks the signal peptide and first Ig-like domain. Siglec-15 associates with the signaling molecules DAP12 and DAP10 (2-5). It is expressed on osteoclasts, macrophages, and dendritic cells (2-6) and binds to the sialyl-Tn antigen (2, 3, 6). This interaction induces the production of TGF-beta by tumor-associated macrophages (3). Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts (4-6).

References

Humphrey, M.B. and M.C. Nakamura (2015) Clin. Rev. Allergy Immunol. PMID 26573914.
Angata, T. et al. (2007) Glycobiology 17:838.
Takamiya, R. et al. (2013) Glycobiology 23:178.
Kameda, Y. et al. (2013) J. Bone Miner. Res. 28:2463.
Stuible, M. et al. (2014) J. Biol. Chem. 289:6498.
Ishida-Kitagawa, N. et al. (2012) J. Biol. Chem. 287:17493.

Long Name

Sialic Acid Binding Ig-like Lectin 15

Alternate Names

CD33L3, HsT1361, Siglec15

Entrez Gene IDs

284266 (Human); 620235 (Mouse); 498888 (Rat)

Gene Symbol

SIGLEC15

UniProt

Q6ZMC9

Additional Siglec-15 Products

Product Documents for Human Siglec-15 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Siglec-15 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human Siglec-15 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9227

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Siglec-15 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human Siglec-15 Antibody

Detection of Siglec-15 in HEK293 Human Cell Line transfected with Human Siglec-15 and eGFP by Flow Cytometry