Human Siglec-3/CD33 (Research Grade Gemtuzumab Biosimilar) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB9635
Recombinant Monoclonal Antibody For Research Use Only
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
CyTOF-ready, Flow Cytometry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Human IgG1 Clone # Hu9a
Product Summary for Human Siglec-3/CD33 (Research Grade Gemtuzumab Biosimilar) Antibody
Immunogen
Human Siglec‑3/CD33
Specificity
Detects human Siglec‑3/CD33 based on Gemtuzumab therapeutic antibody. This non-therapeutic antibody uses the same variable region sequence as the therapeutic antibody Gemtuzumab. This product is for research use only.
Clonality
Monoclonal
Host
Human
Isotype
IgG1
Scientific Data Images for Human Siglec-3/CD33 (Research Grade Gemtuzumab Biosimilar) Antibody
Detection of Siglec-3/CD33 on U937 Human Cell Line by Flow Cytometry.
U937 Human histiocytic lymphoma cell line was stained with Human Anti-Human Siglec-3/CD33 (Research Grade Gemtuzumab Biosimilar) Monoclonal Antibody (Catalog # MAB9635, filled histogram) or irrelevant antibody (open histogram) followed by APC-conjugated Anti-Human IgG Secondary Antibody (Catalog # F0135). View our protocol for Staining Membrane-associated Proteins.Applications for Human Siglec-3/CD33 (Research Grade Gemtuzumab Biosimilar) Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: U937 Human Histiocytic Lymphoma cell line
Sample: U937 Human Histiocytic Lymphoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Siglec-3/CD33
Long Name
Sialic Acid Binding Ig-like Lectin 3
Alternate Names
CD33, gp67, Siglec3
Gene Symbol
CD33
Additional Siglec-3/CD33 Products
Product Documents for Human Siglec-3/CD33 (Research Grade Gemtuzumab Biosimilar) Antibody
Product Specific Notices for Human Siglec-3/CD33 (Research Grade Gemtuzumab Biosimilar) Antibody
For research use only
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