Human SIRP alpha/CD172a
Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4546
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
CyTOF-ready, Flow Cytometry, Immunocytochemistry, Western Blot
Cited:
Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 602411
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human SIRP alpha/CD172a
Gly27-Asn370 (predicted)
Accession # P78324
Gly27-Asn370 (predicted)
Accession # P78324
Specificity
Detects human SIRP alpha/CD172a in direct ELISAs and Western blots. In direct ELISAs, 50-100% cross-reactivity with recombinant human (rh) SIRP beta1 and no cross-reactivity with rhSIRP beta2 is observed. In Western blots, approximately 10% cross-reactivity with rhSIRP beta1 and no cross-reactivity with rhSIRP beta2 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human SIRP alpha/CD172a Antibody
Detection of Human SIRP alpha/CD172a
by Western Blot.
Western blot shows lysates of A549 human lung carcinoma cell line, U2OS human osteosarcoma cell line, U937 human histiocytic lymphoma cell line, and human granulocytes. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human SIRP alpha/CD172aMonoclonal Antibody (Catalog # MAB4546) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SIRP alpha/CD172a
at approximately 70-95 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Western Blot Buffer Group 1.
Detection of SIRP alpha/CD172a in U937 Human Cell Line by Flow Cytometry.
U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human SIRPa/CD172a Monoclonal Antibody (Catalog # MAB4546, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0101B).
SIRP alpha/CD172a in THP‑1 Human Cell Line.
SIRPa/CD172a was detected in immersion fixed THP-1 human acute monocytic leukemia cell line using Mouse Anti-Human SIRPa/CD172a Monoclonal Antibody (Catalog # MAB4546) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI. Specific staining was localized to cell membranes. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human SIRP alpha/CD172a Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: U937 human histiocytic lymphoma cell line
Sample: U937 human histiocytic lymphoma cell line
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed THP‑1 human acute monocytic leukemia cell line
Sample: Immersion fixed THP‑1 human acute monocytic leukemia cell line
Western Blot
2 µg/mL
Sample: A549 human lung carcinoma cell line, U2OS human osteosarcoma cell line, U937 human histiocytic lymphoma cell line, and human granulocytes
Sample: A549 human lung carcinoma cell line, U2OS human osteosarcoma cell line, U937 human histiocytic lymphoma cell line, and human granulocytes
Reviewed Applications
Read 3 reviews rated 4.3 using MAB4546 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SIRP alpha/CD172a
SIRP alpha (Signal regulatory protein alpha; also SHPS1 and BIT) is a variably glycosylated 90-120 kDa member of the SIRP family of proteins. It is widely expressed, being found on neurons, microglia/macrophages, endothelium, and fibroblasts. SIRP alpha has a variety of functions, including presynsptic organization, inhibition of integrin action, and induction of myogenesis. It binds to CD47 and likely other ligands. Mature human SIRP alpha is a 477 amino acid (aa) type I transmembrane glycoprotein. It contains an extracellular region (aa 27-372) that shows one V-type Ig-like (aa 32-137) and two C2-type Ig-like domains (aa 147-347). Its cytoplasmic domain possesses two ITIMs which interact with protein tyrosine phosphatases. There is one alternative start site at Met102 plus a four aa insertion after Gln421. Over aa 27-370, human SIRP alpha shares 61% aa identity with mouse SIRP alpha.
Long Name
Signal-regulatory Protein alpha
Alternate Names
BIT, CD172a, MFR, MYD-1, SHPS1, SIRPA
Entrez Gene IDs
Gene Symbol
SIRPA
UniProt
Additional SIRP alpha/CD172a Products
Product Documents for Human SIRP alpha/CD172a Antibody
Product Specific Notices for Human SIRP alpha/CD172a Antibody
For research use only
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