Human SIRP alpha/CD172a PE-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB4546P
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Flow Cytometry
Label
Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)
Antibody Source
Monoclonal Mouse IgG2B Clone # 602411
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human SIRP alpha/CD172a
Gly27-Asn370 (predicted)
Accession # P78324
Gly27-Asn370 (predicted)
Accession # P78324
Specificity
Detects human SIRP alpha/CD172a in direct ELISAs and Western blots. In direct ELISAs, 50-100% cross-reactivity with recombinant human (rh) SIRP beta1 and no cross-reactivity with rhSIRP beta2 is observed. In Western blots, approximately 10% cross-reactivity with rhSIRP beta1 and no cross-reactivity with rhSIRP beta2 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human SIRP alpha/CD172a PE-conjugated Antibody
Detection of SIRP alpha/CD172a in U937 Human Cell Line by Flow Cytometry.
U937 human histiocytic lymphoma cell line was stained with Mouse Anti-Human SIRPa/CD172a PE-conjugated Monoclonal Antibody (Catalog # FAB4546P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.Applications for Human SIRP alpha/CD172a PE-conjugated Antibody
Application
Recommended Usage
Flow Cytometry
10 µL/106 cells
Sample: U937 human histiocytic lymphoma cell line
Sample: U937 human histiocytic lymphoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: SIRP alpha/CD172a
SIRP alpha (Signal regulatory protein alpha; also SHPS1 and BIT) is a variably glycosylated 90-120 kDa member of the SIRP family of proteins. It is widely expressed, being found on neurons, microglia/macrophages, endothelium, and fibroblasts. SIRP alpha has a variety of functions, including presynsptic organization, inhibition of integrin action, and induction of myogenesis. It binds to CD47 and likely other ligands. Mature human SIRP alpha is a 477 amino acid (aa) type I transmembrane glycoprotein. It contains an extracellular region (aa 27-372) that shows one V-type Ig-like (aa 32-137) and two C2-type Ig-like domains (aa 147-347). Its cytoplasmic domain possesses two ITIMs which interact with protein tyrosine phosphatases. There is one alternative start site at Met102 plus a four aa insertion after Gln421. Over aa 27-370, human SIRP alpha shares 61% aa identity with mouse SIRP alpha.
Long Name
Signal-regulatory Protein alpha
Alternate Names
BIT, CD172a, MFR, MYD-1, SHPS1, SIRPA
Entrez Gene IDs
Gene Symbol
SIRPA
UniProt
Additional SIRP alpha/CD172a Products
Product Documents for Human SIRP alpha/CD172a PE-conjugated Antibody
Product Specific Notices for Human SIRP alpha/CD172a PE-conjugated Antibody
For research use only
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