Human Skp2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6817
Key Product Details
Species Reactivity
Human
Applications
Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 712120
Product Specifications
Immunogen
E. coli-derived recombinant human Skp2
Leu130-Pro350
Accession # Q13309
Leu130-Pro350
Accession # Q13309
Specificity
Detects human Skp2 in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human Skp2 Antibody
Detection of Human Skp2 by Western Blot.
Western blot shows lysates of 293T human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and U2OS human osteosarcoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Skp2 Monoclonal Antibody (Catalog # MAB6817) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). Specific bands were detected for Skp2 at approximately 45 and 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Skp2 by Simple WesternTM.
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cells, loaded at 0.2 mg/mL. A specific band was detected for Skp2 at approximately 49 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Skp2 Monoclonal Antibody (Catalog # MAB6817). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human Skp2 Antibody
Application
Recommended Usage
Simple Western
20 µg/mL
Sample: HeLa human cervical epithelial carcinoma cells
Sample: HeLa human cervical epithelial carcinoma cells
Western Blot
2 µg/mL
Sample: 293T human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and U2OS human osteosarcoma cell line
Sample: 293T human embryonic kidney cell line, HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and U2OS human osteosarcoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 4 using MAB6817 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Skp2
Long Name
S Phase Kinase-associated Protein 2
Alternate Names
FBL1, FBXL1, p45skp2
Gene Symbol
SKP2
UniProt
Additional Skp2 Products
Product Documents for Human Skp2 Antibody
Product Specific Notices for Human Skp2 Antibody
For research use only
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