Detects HEK293 human embryonic kidney cell line transfected with human SLC5A8/SMCT1 by Flow Cytometry. Does not detect untransfected or irrelevant transfected HEK293 cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human SLC5A8/SMCT1 Antibody

Detection of SLC5A8/SMCT1 in MCF‑7 Human Cell Line by Flow Cytometry.

MCF-7 human breast cancer cell line was stained with Mouse Anti-Human SLC5A8/SMCT1 Monoclonal Antibody (Catalog # MAB8398, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Detection of SLC5A8/SMCT1 in HEK293 Human Cell Line Transfected with Human SLC5A8/SMCT1 and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with human SLC5A8/SMCT1 and eGFP was stained with either (A) Mouse Anti-Human SLC5A8/SMCT1 Monoclonal Antibody (Catalog # MAB8398) or (B) Mouse IgG₁Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Applications for Human SLC5A8/SMCT1 Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/10⁶ cells
Sample: MCF‑7 human breast cancer cell line and HEK293 human embryonic kidney cell line transfected with human SLC5A8/SMCT1 and eGFP

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SLC5A8/SMCT1

SLC5A8/SMCT1 (Solute Carrier family 5, member 8) is a member of the solute carrier family of multi-pass membrane proteins. SLC5A8 has been shown to transport iodide by a passive mechanism. It also transports monocarboxylates and short-chain fatty acids by a sodium-coupled mechanism. SLC5A8 nuclear translocation and loss of expression are associated with poor outcome in pancreatic ductal adenocarcinoma (1). It may be responsible for the absorption of D-lactate and monocarboxylate drugs from the intestinal tract.

References

Helm, J. et al. (2012) Pancreas 41:904.

Long Name

Solute Carrier Family 5 Iodide Transporter), Member 8

Alternate Names

AIT, SMCT

Entrez Gene IDs

160728 (Human); 216225 (Mouse); 500820 (Rat)

Gene Symbol

SLC5A8

UniProt

NP_666018

Additional SLC5A8/SMCT1 Products

Product Documents for Human SLC5A8/SMCT1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human SLC5A8/SMCT1 Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human SLC5A8/SMCT1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8398

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

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Product Specifications

Immunogen

Specificity