Human Sonic Hedgehog/Shh Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB10939
Key Product Details
Species Reactivity
Human
Applications
ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 605015
Product Specifications
Immunogen
E. coli-derived human Sonic Hedgehog/Shh protein
Cys24-Gly197
Accession # NP_000184.1
Cys24-Gly197
Accession # NP_000184.1
Specificity
Detects human Sonic Hedgehog/Shh in direct ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human Sonic Hedgehog/Shh Antibody
Human Sonic Hedgehog/Shh ELISA Standard Curve.
Recombinant Human Sonic Hedgehog/Shh protein was serially diluted 2-fold and captured by Mouse Anti-Human Sonic Hedgehog/Shh Monoclonal Antibody (Catalog # MAB10939) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Sonic Hedgehog/Shh Monoclonal Antibody (Catalog # MAB109391) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).Applications for Human Sonic Hedgehog/Shh Antibody
Application
Recommended Usage
ELISA
This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human Sonic Hedgehog/Shh Monoclonal Antibody (Catalog # MAB109391).
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human Sonic Hedgehog/Shh N-Terminus DuoSet ELISA Kit (Catalog # DY1314-05) for convenient development of a sandwich ELISA.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Sonic Hedgehog/Shh
N-terminal cysteine and modified by cholesterol addition at its C-terminus (6). These modifications contribute to the membrane tethering of Shh as well as its assembly into various sized multimers (6-9). Lipid modification and multimerization greatly increase Shh-N receptor binding affinity and signaling potency (5, 6, 8, 9). Monomeric and multimeric Shh can be released from the plasma membrane by the cooperative action of DISP1, SCUBE2, and TACE/ADAM17 (10-12). Modifications also extend the effective range of Shh functionality and are required for the development of protein gradients important in tissue morphogenesis (9, 13). Canonical signaling of Shh is mediated by a multicomponent receptor complex that includes Patched (PTCH1, PTCH2) and Smoothened (SMO) (14). The binding of Shh to PTCH releases the basal repression of SMO by PTCH. Shh activity can also be regulated through interactions with heparin, glypicans, and membrane-associated Hip (hedgehog interacting protein) (13, 15, 16).
References
- Briscoe, J. and P.P. Therond (2013) Mol. Cell. Biol. 14:416.
- Aviles, E.C. et al. (2013) Front. Cell. Neurosci. 7:86.
- Xie, J. et al. (2013) OncoTargets Ther. 6:1425.
- Marigo, V. et al. (1995) Genomics 28:44.
- Zeng, X. et al. (2001) Nature 411:716.
- Feng, J. et al. (2004) Development 131:4357.
- Goetz, J.A. et al. (2006) J. Biol. Chem. 281:4087.
- Pepinsky, R.B. et al. (1998) J. Biol. Chem. 273:14037.
- Chen, M.-H. et al. (2004) Genes Dev. 18:641.
- Etheridge, L.A. et al. (2010) Development 137:133.
- Jakobs, P. et al. (2014) J. Cell Sci. 127:1726.
- Dierker, T. et al. (2009) J. Biol. Chem. 284:8013.
- Lewis, P.M. et al. (2001) Cell 105:599.
- Carpenter, D. et al. (1998) Proc. Natl. Acad. Sci. USA 95:13630.
- Filmus, J. and M. Capurro (2014) Matrix Biol. 35:248.
- Chuang, P.-T. and A.P. McMahon (1999) Nature 397:617.
Alternate Names
HHG1, HLP3, HPE3, MCOPCB5, Shh, ShhNC, SMMCI, TPTPS
Gene Symbol
SHH
UniProt
Additional Sonic Hedgehog/Shh Products
Product Documents for Human Sonic Hedgehog/Shh Antibody
Product Specific Notices for Human Sonic Hedgehog/Shh Antibody
For research use only
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